RT-LAMP · Cat. no. HW206-P01
AcuGenix™ Hot Start RT-LAMP Probe Master Mix-V2
AcuGenix™ Hot Start RT-LAMP Probe Master Mix-V2, HW206-P01, is supplied for molecular diagnostic research and manufacturing workflows.
Glycerol-free
Compatible with lyophilization
Engineered Bst HS
Faster amplification, higher yield than wild type
15 freeze-thaw cycles
Stable amplification
Product overview
AcuGenix™ Hot Start RT-LAMP Probe Master Mix-V2 adopts the probe-based isothermal amplification method and can be used for qualitative detection of RNA in samples (e.g., viruses). AcuGenix™ Bst Hot Start DNA Polymerase-V2 is derived from the DNA polymerase of Bacillus stearothermophilus.
Available versions
| Cat. no. | Product name | Formulation |
|---|---|---|
| HW206-P01 | AcuGenix™ Hot Start RT-LAMP Probe Master Mix-V2 | Liquid (with glycerol) |
| FHW1008-P01 | AcuGenix™ Lyo-ready Hot Start RT-LAMP Probe Master Mix-V2 | Lyo-ready |
Features and applications
Features
- Designed for probe-based LAMP detection of RNA samples, requiring instrumentation for fluorescence readout.
- Reactions can be assembled at room temperature, effectively preventing non-specific amplification and enhancing reaction efficiency.
- Optimized recombinant hot-start polymerase with excellent amplification performance. The genetically engineered and reversibly modified Bst hot start DNA polymerase greatly improves amplification speed and product yield compared with wild-type enzymes, and has strong strand displacement activity suitable for LAMP reactions.
- Glycerol-free formula compatible with lyophilization. The premix contains no glycerol, which is perfectly suitable for the development of freeze-dried products and meets the production stability requirements of lyophilized diagnostic reagents.
Applications
- Qualitative detection of RNA from samples including viruses by probe-based RT-LAMP.
- Preparation of all-in-one lyophilized LAMP products (lyophilized beads, in-situ lyophilized powder).
Components
| Component | Specification/Notes |
|---|---|
| Liquid (with glycerol), HW206-P01 | |
| AcuGenix™ Bst Hot Start DNA Polymerase-V2 (8 U/µL) | N/A |
| 2×RT-LAMP Premix Buffer Ⅱ | Included component; refer to the instruction manual |
| Neoscript RTase (200 U/µL) | Included component; refer to the instruction manual |
| RNase HⅡ (50 mU/μL) | Included component; refer to the instruction manual |
| Lyo-ready, FHW1008-P01 | |
| 5×RT-LAMP Lyophilization Buffer | 5× |
| 10×RT-LAMP Premix (DG) (Bst2.0 HS) (RNase HⅡ) | 10× |
Performance data
1. Freeze-Thaw Stability Test
Amplification Curves
| Store at -20°C 5 freeze-thaw cycles 10 freeze-thaw cycles 15 freeze-thaw cycles | ||||
| channel | 1ng/T | 0.1ng/T | 0.01ng/T | NTC |
|---|---|---|---|---|
| FAM |  |  |  |  |
Conclusion 1: The FHW1008-P01 sample demonstrates stable amplification after 5, 10, or 15 freeze-thaw cycles.
Shipping and storage
| Condition | Recommendation |
|---|---|
| Shipping Conditions | -20±5°C |
| Storage Conditions | -20±5°C |
| Shelf Life | 24M |
Downloads
AcuGenix™ Hot Start RT-LAMP Probe Master Mix-V2 (HW206-P01)
Instruction Manual (PDF)COA (PDF)MSDS 1 (PDF)MSDS 2 (PDF)MSDS 3 (PDF)MSDS 4 (PDF)AcuGenix™ Lyo-ready Hot Start RT-LAMP Probe Master Mix-V2 (FHW1008-P01)
Instruction Manual (PDF)COA (PDF)MSDS 1 (PDF)MSDS 2 (PDF)
Need this product lyophilized, in bulk, or in a custom format?
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