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AcuGenix™ Hot Start Taq DNA Polymerase (Glycerol-Free), FE03, is a chemically modified enzyme optimized for lyophilized PCR and qPCR reagent development.
AcuGenix™ Hot Start Taq DNA Polymerase (Glycerol-Free), FE03
USD
$77.00
FE03: FE03-1
FE03: FE03-1
Product information published by Zhuhai Biori Biotechnology Co., Ltd.
Features
- Glycerol-free storage formulation optimized for lyophilized PCR and qPCR reagents.
- Chemically modified hot-start enzyme with activity blocked at room temperature.
- Time-release activation during PCR temperature ramping supports specific amplification.
- Helps suppress non-specific amplification caused by primer mis-annealing and primer dimers.
- Designed for high sensitivity, strong fluorescence signals, and high specificity in real-time PCR.
- Suitable for multiplex PCR and amplification of low-copy templates.
- Provides 5'-3' polymerase and 5'-3' exonuclease activities; lacks 3'-5' proofreading activity.
- Generates PCR products with a 3' A overhang for direct T-vector cloning.
Application
- Development of lyophilized endpoint PCR and qPCR reagents.
- Conventional PCR and endpoint PCR.
- Real-time PCR (qPCR) and qRT-PCR workflows after reverse transcription.
- Multiplex PCR and low-copy template amplification.
- Probe-based and fluorescent dye-based assays.
- Gene chip assays and other molecular detection workflows requiring hot-start amplification.
Components
| Component | Specification / Notes |
|---|---|
| AcuGenix™ Hot Start Taq DNA Polymerase (Glycerol-Free) | 5 U/µL |
| 10× PCR Buffer II (Mg²⁺ free) (optional) | Does not contain dNTPs or Mg²⁺. |
| MgCl2 (optional) | 25 mM |
Shipping and Storage
| Condition | Recommendation |
|---|---|
| Shipping Temperature | Cold-chain shipment under conditions compatible with the stated frozen storage requirement. |
| Storage Temperature | Store at -20 ± 5°C. Vortex before use and avoid repeated freeze-thaw cycles. |
Data
Coming soon.
