Taq DNA Polymerase · Cat. no. FE03-NA
AcuGenix™ Hot Start Taq DNA Polymerase-Low DNA (Glycerol-Free)
AcuGenix™ FE03-NA is a glycerol-free, low-residual-DNA Hot Start Taq DNA Polymerase for lyophilized PCR/qPCR and biopharmaceutical reagent development.
Glycerol-free
For lyophilized reagent development
Low residual DNA
Processed for biopharma detection reagents
Chemical hot start
Blocked at room temperature
Product overview
AcuGenix™ FE03-NA is a glycerol-free, low-residual-DNA Hot Start Taq DNA Polymerase for lyophilized PCR/qPCR and biopharmaceutical reagent development.
Features and applications
Features
- Combines a glycerol-free storage formulation with special low residual DNA processing.
- Optimized for lyophilized PCR and qPCR reagent development.
- Designed for biopharmaceutical detection-reagent development where reagent-derived DNA background is a concern.
- Chemically modified hot-start enzyme with activity blocked at room temperature.
- Time-release activation during PCR temperature ramping supports specific amplification.
- Helps suppress non-specific amplification caused by primer mis-annealing and primer dimers.
- Designed for high sensitivity, strong fluorescence signals, and high specificity in real-time PCR.
- Suitable for multiplex PCR and amplification of low-copy templates.
- Provides 5'-3' polymerase and 5'-3' exonuclease activities; lacks 3'-5' proofreading activity.
- Generates PCR products with a 3' A overhang for direct T-vector cloning.
Applications
- Development of lyophilized biopharmaceutical detection reagents requiring glycerol-free and low residual DNA positioning.
- Conventional PCR and endpoint PCR.
- Real-time PCR (qPCR) and qRT-PCR workflows after reverse transcription.
- Multiplex PCR and low-copy template amplification.
- Probe-based and fluorescent dye-based assays.
- Gene chip assays and other molecular detection workflows requiring hot-start amplification.
Components
| Component | Specification / Notes |
|---|---|
| AcuGenix™ Hot Start Taq DNA Polymerase-Low DNA (Glycerol-Free) | 5 U/µL |
| 10× PCR Buffer II (Mg²⁺ free) (optional) | Does not contain dNTPs or Mg²⁺. |
| MgCl2 (optional) | 25 mM |
Shipping and storage
| Condition | Recommendation |
|---|---|
| Shipping Temperature | Cold-chain shipment under conditions compatible with the stated frozen storage requirement. |
| Storage Temperature | Store at -20 ± 5°C. Vortex before use and avoid repeated freeze-thaw cycles. |
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