AcuGenix™ phi29 DNA Polymerase

High-Fidelity, Long-Range Isothermal DNA Polymerase for Advanced Genomic Workflows

> 70 kb

Single binding event

Strand displacement

RCA and MDA workflows

0.1 ng gDNA

Higher RCA yield than competitor

Product overview

AcuGenix™ phi29 DNA Polymerase is a replicative DNA polymerase derived from Bacillus subtilis bacteriophage phi29, recombinantly expressed in a validated Escherichia coli strain carrying the native phi29 polymerase gene. It is engineered for high-performance isothermal amplification workflows, eliminating the need for thermal cyclers while delivering exceptional long-range synthesis capability and superior replication fidelity.

Features and applications

Features

Robust Strand-Displacement Activity: Efficiently displaces downstream DNA strands without helicase assistance, enabling core isothermal workflows including rolling-circle amplification (RCA) and multiple displacement amplification (MDA).
Ultra-High Processivity: Synthesizes DNA fragments exceeding 70 kb in a single binding-elongation event, ideal for large-genome or complex repeat-region amplification.
Intrinsic Proofreading for High Fidelity: Possesses native 3’→5’ exonuclease activity that corrects misincorporated nucleotides, delivering superior replication accuracy for downstream sequencing and cloning applications.
True Isothermal Operation: Functions efficiently at a constant temperature, removing reliance on thermal cyclers and reducing equipment costs for resource-limited labs or point-of-care settings.
Consistent Recombinant Quality: Produced via a rigorously controlled recombinant expression system, ensuring excellent batch-to-batch consistency, high purity, and minimal nuclease contamination.

Applications

Genome Research: Whole-genome amplification (WGA) for single-cell, circulating cell-free DNA (cfDNA), or low-input samples; metagenomic library preparation; long-range DNA template generation
Molecular Cloning & Plasmid Production: In-vitro plasmid preparation; amplification of long or GC-rich gene fragments for cloning
Diagnostic Assay Development: SNP genotyping; isothermal nucleic acid detection assay development; low-copy pathogen detection workflows

Components

Components	Specification/Notes
10×phi29 Buffer	10×
phi29 DNA Polymerase	10 U/ μL

Performance data

Amplification Efficiency

Note: BR3P104 is the catalog number. The 0.1 ng input corresponds to ~15 diploid human genomic copies, demonstrating robust performance for trace-sample applications including single-cell genomics and rare pathogen detection without the need for pre-amplification.