AcuGenix™ RNA Fast Plant Extraction Kit

11-Minute High-Purity RNA Isolation for Challenging Plant Tissues – No Phenol, No DNase, No Compromise

11 min

Extraction after homogenization

No phenol-chloroform

No β-ME, DTT or DNase I needed

20 plant tissues

Yield and purity comparable to Supplier A

AcuGenix™ RNA Fast Plant Extraction Kit, BR2B202 product image

Product overview

The AcuGenix™ RNA Fast Plant Extraction Kit is engineered for the rapid isolation of high-quality total RNA from 50-150 mg of diverse plant tissues, including samples from common species as well as polyphenol- and polysaccharide-rich tissues (roots, stems, leaves, flowers, fruits, seeds, etc.). It is optimized for plant molecular biology workflows requiring reliable, inhibitor-free RNA input.

Features and applications

Features

Broad Compatibility with Challenging Tissues: Validated for both standard and recalcitrant plant samples with high levels of secondary metabolites (polyphenols, polysaccharides) that typically compromise RNA quality.
Safe, Toxic-Reagent-Free Workflow: Eliminates the need for hazardous phenol-chloroform extraction, β-mercaptoethanol, DTT, and DNase I digestion, reducing operator exposure risk and waste disposal burden.
Ultra-Rapid 11-Minute Protocol: The entire extraction workflow is completed in just 11 minutes following sample homogenization, drastically reducing hands-on time compared to conventional column- or phenol-based methods.
Efficient gDNA and Impurity Removal: The included gDNA-Filter Column removes genomic DNA and cellular impurities, while the Pure RNA Column I ensures high-efficiency RNA binding for consistent yield.
Exceptional RNA Purity & Integrity: Rapid lysis suppresses nucleases released during cell disruption to preserve RNA integrity. Isolated RNA is free of protein, genomic DNA, and other contaminants that interfere with downstream assays.
Culture Cell Compatibility: Also validated for high-purity RNA extraction from plant cell cultures, supporting diverse experimental models.
Seamless Downstream Compatibility: Purified RNA requires no additional cleanup and is directly compatible with the full AcuGenix™ line of reverse transcription and NGS library preparation kits.

Applications

Gene expression analysis via RT-PCR and quantitative real-time RT-PCR (qRT-PCR)
Transcriptome profiling and RNA-seq library construction
Plant pathogen detection and viroid RNA isolation
Northern blotting and RNA structure analysis
In vitro translation and protein expression studies
Molecular cloning and gene functional analysis
Microarray-based gene expression profiling
Non-model plant genomics and molecular breeding research

Components

Components	Specification/Notes
PLB Reagent	30 mL
Buffer RWA 1	9.6 mL
Buffer RWB 1	12 mL
Proteinase K	0.5 mL
RNase-free ddH₂O	10 mL
gDNA-Filter Columns (each in a 2 mL Collection Tube)	50 T
Pure RNA Columns I (each in a 2 mL Collection Tube)	50 T
RNase-free Collection Tubes 1.5 mL	50 T

Performance data

Broad Sample Compatibility

BR2B202 AcuGenix™ RNA Fast Plant Extraction Kit: Figure 1 Comparative RNA extraction performance across 20 plant sample types vs. leading competitor (Supplier A) — **Figure 1 Comparative RNA extraction performance across 20 plant sample types vs. leading competitor (Supplier A).** BR2B202 (AcuGenix™ RNA Fast Plant Extraction Kit) delivered nucleic acid yield and purity comparable to Supplier A across a diverse panel of plant tissues in validation tests.

High-quality extracted RNA

BR2B202 AcuGenix™ RNA Fast Plant Extraction Kit: Figure 2 RNA quality benchmark — **Figure 2 RNA quality benchmark.** Across 20 sample types—including leaves, roots, fruits, and seeds—BR2B202 outperformed Supplier A in RNA integrity, with zero detectable degradation or genomic DNA contamination. The extracted RNA is ideally suited for downstream applications including plant pathogen surveillance, transcriptome sequencing, and molecular breeding programs.

Note: The absence of genomic DNA contamination eliminates the need for post-extraction DNase treatment, avoiding RNA degradation risks; high-integrity RNA ensures accurate quantification and reliable data for qPCR and RNA-seq workflows.

Extract Performance/Target Amplification Performance

BR2B202 AcuGenix™ RNA Fast Plant Extraction Kit: Figure 3 Comparison of target Ct values via qPCR benchmarking — **Figure 3 Comparison of target Ct values via qPCR benchmarking.** BR2B202 delivered lower Ct values than Supplier A across the majority of tested plant species, reflecting superior RNA quality and downstream amplification efficiency. For key horticultural crops including orange, spinach, and strawberry, BR2B202 showed a clear performance advantage; only rice samples yielded marginally lower Ct values with Supplier A, with BR2B202 maintaining an overall performance edge for plant molecular research.

Note: Lower Ct values indicate higher RNA integrity and purity, with fewer PCR inhibitors and better template availability; these results confirm BR2B202 supports robust gene expression analysis and variant detection workflows in plant research.