Bsa I GMP-grade

GMP-grade Bsa I restriction enzyme for mRNA and IVT template preparation.

20 U/µL

Type IIS, 5′ overhang outside recognition site

0.05 U

Digests 1 µg plasmid in 50 µL

7 days at 37 °C

Stable activity

Product overview

Bsa I GMP-grade, GMP-BP-E10, is a GMP-grade type IIS restriction endonuclease. Its Type IIS cleavage mechanism can generate a "scarless" template, ensuring that the IVT product contains no extra nucleotides. It recognizes the Bsa I site and supports plasmid linearization or preparation of DNA fragments with defined sticky ends.

Available versions

Cat. no.	Product name	Formulation
GMP-BP-E10-2K	Bsa I GMP-grade
GMP-BP-E10-20K	Bsa I GMP-grade
GMP-BP-E10-200K	Bsa I GMP-grade

Features and applications

Features

GMP-grade Bsa I type IIS restriction endonuclease.
(1) Type IIS restriction endonuclease; recognition sequence:
5' ...GGTCTC(N)↓...3'
3' ...CCAGAG(NNNNN)↑...5'
Cleavage sites are located outside the recognition sequence, producing 5′ overhangs.
Activity: 20 U/μL.
Supplied with 10× Bsa I Reaction Buffer.
Quality control: protein purity ≥95%; free of exogenous DNase, RNase, exonuclease, and endonuclease activity; residual host-cell DNA ≤100 pg/mg; residual host-cell protein <50 ppm; bacterial endotoxin <5 EU/mL.

Applications

Plasmid digestion for linearized DNA fragment preparation.
DNA digestion to generate specific sticky ends.
mRNA and IVT template-preparation workflows.

Components

Component	GMP-BP-E10-2K	GMP-BP-E10-20K	GMP-BP-E10-200K
Bsa I GMP-grade (20 U/μL)	0.1 mL / 2 KU	1 mL / 20 KU	10 mL / 200 KU
10× Bsa I Reaction Buffer	1 mL	5 mL	50 mL

Performance data

1 Digestion of plasmid by varying units of Bsa I GMP-grade

Description: To evaluate the digestion performance of Bsa I GMP-grade on 1 μg of plasmid containing a single Bsa I recognition site, using varying enzyme amounts.

GMP-BP-E10 Bsa I GMP-grade: Figure 1 Digestion of plasmid by increasing units of Bsa I GMP-grade — **Figure 1** Digestion of plasmid by increasing units of Bsa I GMP-grade

Conclusion: Bsa I GMP-grade demonstrated robust digestion activity. The results showed that 0.05 U of Bsa I GMP-grade was sufficient to completely digest 1 μg of plasmid template in a standard 50 μL reaction system.

2 37°C Accelerated Stability

Description: To evaluate the thermal stability of Bsa I GMP-grade after incubation at 37°C for 7 days. Residual digestion activity of Bsa I GMP-grade was measured after storage at 37°C for 1, 3, 5, and 7 days, respectively.

GMP-BP-E10 Bsa I GMP-grade: Figure 2 Activity of Bsa I GMP-grade after 1, 3, 5, and 7 days at 37°C. — **Figure 2** Activity of Bsa I GMP-grade after 1, 3, 5, and 7 days at 37°C.

Conclusion: Bsa I GMP-grade maintained stable activity throughout the 7-day incubation period at 37°C, with no significant loss of digestion performance compared with the enzyme stored at -20°C.