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Product Catalog
GMP-grade Bsa I type IIS restriction endonuclease for precise DNA digestion, sticky-end generation, and linearized fragment preparation in molecular biology and biopharma workflows.
Features
- Type IIS restriction endonuclease recognizing 5′-GGTCTC(N)↓-3′ / 3′-CGAGAG(NNNN)↑-5′
- Efficient digestion for generation of specific sticky ends
- Suitable for preparing linearized DNA fragments with poly(A/T/G/C) ends
- 20 U/μL activity for reliable restriction digestion workflows
- Supplied with dedicated 10× Bsa I Reaction Buffer
- GMP-grade manufacturing with protein purity ≥95%
- Free of exogenous DNase, RNase, exonuclease, and endonuclease activity
- Low residual host-cell DNA, low endotoxin, and controlled impurity profile
Application
- Restriction digestion of plasmids to prepare linearized DNA fragments
- Generation of defined sticky ends for cloning, DNA assembly, and construct preparation
- Preparation of DNA substrates for downstream transcription or template engineering workflows
- Molecular biology and biopharma process development workflows requiring controlled DNA cleavage
Components
| No. | Component | GMP-BP-E10-2K | GMP-BP-E10-20K | GMP-BP-E10-200K |
|---|---|---|---|---|
| 1 | Bsa I GMP-grade | 2 KU / 0.1 mL | 20 KU / 1 mL | 200 KU / 10 mL |
| 2 | 10× Bsa I Reaction Buffer | 1 mL | 5 mL | 50 mL |
Shipping and Storage
| Condition | Recommendation |
|---|---|
| Storage | Store at -20 ± 5 °C. |
| Handling | Prepare reaction mix on ice. Thaw and mix thoroughly before use. Avoid repeated freezing and thawing. |
| Digest loading | Final restriction digest content should not exceed 1/10 of the total reaction volume. |
| Storage Buffer | 10 mM Tris-HCl, 200 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 200 μg/mL HSA, 50% (v/v) glycerol, pH 7.4 at 25 °C. |
Data
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