DNase Residual Quantitative Detection Kit (Fluorescent Probe Method)

Deoxyribonuclease (DNase) is an enzyme capable of hydrolyzing the phosphodiester bonds in DNA molecules. Similar to ribonuclease (RNase), DNase is widely present in laboratory environments and biological materials. Since nucleases can degrade nucleic acids, their presence can interfere with many experiments, making it necessary to assay for DNase. The DNase Residual Quantitative Detection Kit independently developed and launched by BIORI utilizes the principle of fluorescent probe technology, enabling rapid quantitative detection of DNase with advantages such as high sensitivity and simple operation. The DNase substrate used in this kit is a synthetic DNA oligonucleotide probe. One end of the probe is labeled with a VIC fluorophore (also called the donor), and the other end is labeled with a BHQ1 quencher (also called the acceptor). When the distance between these two fluorescent groups is appropriate, fluorescence energy is transferred from the donor to the acceptor, resulting in the attenuation of the donor fluorophore's own fluorescence intensity. When this substrate is cleaved by DNase, the two ends of the DNA substrate separate, causing the two groups to move apart. The VIC fluorescence is no longer quenched by BHQ1, allowing the detection of VIC fluorescence. The rate of increase in the fluorescence signal is positively correlated with the amount and activity of the enzyme.