mRNA QC Tools · Cat. no. BP-F15-50
Lyo-ready RNA Ladder 12000, BP-F15
Lyophilized RNA ladder for 1000-12000 nt sizing.
Lyo-ready
Room-temperature shippable ladder
25 °C · 14 days
Bands intact after 10 openings
Product overview
Lyo-ready RNA Ladder 12000, BP-F15, is a premixed lyophilized RNA molecular weight standard for estimating RNA fragment size. It contains reference bands at 1000, 2000, 4000, 6000, 8000, 10000, and 12000 nt.
Features and applications
Features
- Premixed RNA molecular weight standard prepared by in vitro transcription of seven linear DNA templates followed by mixing, purification, and lyophilization.
- Seven clearly identifiable reference bands: 1000, 2000, 4000, 6000, 8000, 10000, and 12000 nt.
- Can be used for 1-1.5% TAE/TBE agarose gel electrophoresis and 1% formaldehyde denaturing agarose gel electrophoresis.
- Stored in a lyophilized state at 2-8°C before reconstitution.
Applications
- Estimating the sizes of RNA fragments by gel electrophoresis.
- Reference standard for linear single-stranded RNA molecules.
Components
| Components | Cat. No. | Volume |
|---|---|---|
| Lyo-ready RNA Ladder 12000 | BP-AS-32 (DG) | Lyophilized ×5 tubes |
| Ladder complex solution | BP-AS-113 | 100 μL |
| 2× Loading Dye | BP-AS-22 | 100 μL |
Performance data
1 Product Stability test
Description: Verify the storage stability and stability upon opening of the Lyo-ready RNA Ladder 12000 product.
(1: control; 2: 25°C for 14 days)
(1: control; 2: 10 repeated lid-opening cycles)
Conclusion: After storage at 25°C for 14 days and 10 repeated opening-and-use cycles, the bands of Lyo-ready RNA Ladder 12000 remained clearly visible by agarose gel electrophoresis, with no obvious degradation observed.
Shipping and storage
| Condition | Recommendation |
|---|---|
| Storage | Store in a lyophilized state at 2-8°C, and store the reconstituted liquid at -70±10°C. |
| Shipping | Ship lyophilized product at 2-8°C. If transported after reconstitution, maintain frozen conditions at -70±10°C. |
| Handling | Avoid repeated freeze-thaw cycles; mix thoroughly after thawing where applicable. |
Downloads
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