HEK293 Host Cell Protein (HCP) ELISA Kit

Quantifies HEK293 HCP in bioproduct samples by sandwich ELISA.

4–200 ng/mL

Standard curve range

2 ng/mL

Limit of detection

CV < 10%

Intra-batch, inter-batch < 15%

Product overview

HEK293 Host Cell Protein (HCP) ELISA Kit, BP-QP-H005, is a 96 T/Kit double-antibody sandwich ELISA kit for quantifying HEK293, HEK293F, and HEK293T host cell protein in bioproduct samples. The manual states a 4-200 ng/mL standard curve range, a 2 ng/mL detection limit, intra-batch CV below 10%, inter-batch CV below 15%, and recovery between 80% and 120%.

Features and applications

Features

ELISA double-antibody sandwich assay format.
Supplied as 96 T/Kit.
This kit has been fully evaluated, with a standard curve range of 4-200 ng/mL and a detection limit of 2 ng/mL. The intra-batch CV is less than 10%, inter-batch CV is less than 15%; the recovery rates are between 80% and 120%.
For research use only and not intended for medical diagnosis, as stated in the manual.

Applications

This kit is used for the quantification of HEK293, HEK293F and HEK293T host cell protein (HCP) in bio-products expressed and amplified using HEK293 strains. This kit is for research and manufacturing use only and is not intended for medical diagnosis.
Designed for HCP quantification in bioproduct samples from the named expression system.

Components

Box	Component	Specification	Storage temperature
Box 1	HEK293 HCP standard (12.5 μg/mL)	30 μL	-20±5°C
Box 2	HEK293 HCP detection antibody	12 mL	2-8°C
	Concentrated Wash Buffer (20×)	25 mL
	Sample Diluent	25 mL
	TMB Substrate	12 mL
	Stop Solution	6 mL
	Anti-HEK293 HCP coated microplate	12 × 8-well strips
	Sealing film	2 sheets
	Instruction Manual	1 copy

Shipping and storage

Condition	Recommendation
Storage	Box 1 (HEK293 HCP standard): -20±5°C. Box 2 (all other components): 2-8°C. The shelf life of an unopened product is 12 months. After opening, the product is valid for 6 months (within the expiration date). After use, return the kit promptly to its specified storage conditions.
Shipping	Ship at 2-8°C with refrigerated cold packs.
Handling	For research use only. Review the MSDS before use.