MDCK Host Cell Protein (HCP) ELISA Kit

Quantifies MDCK HCP in bioproduct samples by sandwich ELISA.

3–200 ng/mL

Standard curve range

3 ng/mL

Limit of quantitation

CV < 10%

Intra-batch, inter-batch < 15%

Product overview

MDCK Host Cell Protein (HCP) ELISA Kit, BP-QP-M006, is a 96 T/Kit double-antibody sandwich ELISA kit for quantifying host cell protein in bioproducts expressed or amplified using MDCK strains. The manual states a 3-200 ng/mL standard curve range, a 3 ng/mL quantitation limit, intra-batch CV below 10%, and inter-batch CV below 15%.

Features and applications

Features

ELISA double-antibody sandwich assay format.
Supplied as 96 T/Kit.
This kit has been fully evaluated, with a standard curve range of 3-200 ng/mL and a quantitation limit of 3 ng/mL. The intra-batch CV is less than 10%, inter-batch CV is less than 15%.
For research use only and not intended for medical diagnosis, as stated in the manual.

Applications

This kit is used for the quantification of host cell protein (HCP) in bio-products expressed and amplified using MDCK strains. This kit is for research use only and is not intended for medical diagnosis.
Designed for HCP quantification in bioproduct samples from the named expression system.

Components

Component	Specification	Storage temperature
MDCK HCP Standards (0, 3, 6, 12, 25, 50, 100, 200 ng/mL)	8 × 1 mL/vial	2-8°C
MDCK HCP detection antibody	12 mL
Concentrated Wash Buffer (20×)	30 mL
Sample Diluent	30 mL
TMB Substrate	12 mL
Stop Solution	6 mL
Anti-MDCK HCP coated microplate	12 × 8-well strips
Sealing film	2 sheets
Instruction Manual	1 copy

Shipping and storage

Condition	Recommendation
Storage	Store kit components at 2-8°C. After use, return the kit to 2-8°C; unopened shelf life is 12 months unless the label states otherwise.
Shipping	Ship at 2-8°C with refrigerated cold packs.
Handling	For research use only. Review the MSDS before use.