Mycoplasma DNA Detection Kit (qPCR), BP-QN22, is a fluorescent probe qPCR kit for qualitative detection of Mycoplasma contamination in biological materials. The assay targets conserved Mycoplasma 16S rRNA regions and includes an internal control to support workflow monitoring.
Mycoplasma DNA Detection Kit (qPCR), BP-QN22
Product information published by Zhuhai Biori Biotechnology Co., Ltd.
Features
- qPCR probe-based assay for conserved Mycoplasma 16S rRNA sequences.
- Internal control can be added during extraction or amplification to monitor extraction efficiency or inhibition.
- Contains UDG enzyme to help prevent contamination from amplified products.
- When used with Biori nucleic acid extraction or purification kits, the manual states a detection limit up to 5 CFU/mL.
- COA reports conforming negative concordance, positive concordance, LOD, and internal-standard Ct results.
Application
- Qualitative Mycoplasma DNA contamination testing.
- Testing of cell cultures, animal sera, and related biological materials.
- qPCR workflows using supported real-time PCR systems listed in the manual.
- Sample extraction workflows paired with a suitable nucleic acid extraction or purification kit.
Components
| Component | Specification |
|---|---|
| My qPCR MIX | 1.0 mL x 1 tube |
| My Positive Control | 1.5 mL x 2 tubes |
| My Negative Control | 1.5 mL x 2 tubes |
| My Internal Control | 1.0 mL x 1 tube |
| Instructions | 1 copy |
Shipping and Storage
| Condition | Recommendation |
|---|---|
| Storage | <= -20 C, protected from light. Shelf life: 24 months. Avoid more than 10 freeze-thaw cycles. |
| Shipping | Ship frozen on dry ice or with validated frozen cold-chain packaging that maintains the specified frozen storage condition. |
| Handling | Protect from light and avoid unnecessary freeze-thaw cycles where applicable. |
Data
Performance Data
Mycoplasma DNA Detection Kit (qPCR) | Catalog No. BP-QN22-50
A product-specific validation study evaluated analytical sensitivity in a cellular matrix and performance across commonly used media and matrices. The selected data below focus on low-level detection and matrix compatibility.
Key Performance at a Glance
| Perf |
Sele |
|---|---|
| Limi |
5 CFU/ |
| Eval |
10 stra |
| Stud |
Lot, inst |
| Free |
Thro |
Analytical Sensitivity
Observed in this validation study: at 5 CFU/mL in a 10^6 cells/mL matrix, each of the 10 evaluated strains produced 24 positive results in 24 replicates.
| Stra |
Posi |
Stra |
Posi |
|---|---|---|---|
| A. laid |
24/ |
M. gall |
24/ |
| M. ferm |
24/ |
M. syno |
24/ |
| M. hyor |
24/ |
M. argi |
24/ |
| M. oral |
24/ |
S. citr |
24/ |
| M. pneu |
24/ |
M. sali |
24/ |
Matrix Interference
The assay was evaluated with the source-listed culture media and matrices. The selected results retain both FAM and VIC measurements and the study controls.
| Samp |
FAM Rep. 1 | FAM Rep. 2 | FAM Mean | VIC Rep. 1 | VIC Rep. 2 | VIC Mean |
|---|---|---|---|---|---|---|
| RPMI 1640 + Myco |
27.44 | 27.39 | 27.42 | 30.30 | 30.08 | 30.19 |
| 10% FBS + Myco |
27.49 | 27.34 | 27.41 | 30.14 | 30.14 | 30.14 |
| PBS + Myco |
27.35 | 27.36 | 27.35 | 30.22 | 29.84 | 30.03 |
| 10% hors |
27.52 | 26.89 | 27.20 | 30.01 | 29.76 | 29.89 |
| Myco |
27.60 | 27.48 | 27.54 | 29.73 | 29.99 | 29.86 |
| M. argi |
27.29 | 27.50 | 27.39 | 29.95 | 29.75 | 29.85 |
| DMEM + Myco |
27.43 | 27.10 | 27.26 | 30.17 | 29.79 | 29.98 |
| AIM- |
27.24 | 27.28 | 27.26 | 29.87 | 29.99 | 29.93 |
| NCS | No Ct | No Ct | 30.00 | 30.17 | 30.08 | |
| NTC | No Ct | No Ct | 30.13 | 30.21 | 30.17 | |
| PC | 26.34 | 26.35 | 26.35 | 30.09 | 30.36 | 30.22 |
Study Scope and Limitations
Results apply to the evaluated strains, cell concentration, matrices, instruments, and controls. This selected upload version does not make a universal species-coverage claim. Users should verify suitability with their own sample matrix and workflow.
