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Product Catalog
Mycoplasma DNA Detection Kit (qPCR), BP-QN22, is a fluorescent probe qPCR kit for qualitative detection of Mycoplasma contamination in biological materials. The assay targets conserved Mycoplasma 16S rRNA regions and includes an internal control to support workflow monitoring.
Mycoplasma DNA Detection Kit (qPCR), BP-QN22
USD
$0.00
BP-QN22-50
Features
- qPCR probe-based assay for conserved Mycoplasma 16S rRNA sequences.
- Internal control can be added during extraction or amplification to monitor extraction efficiency or inhibition.
- Contains UDG enzyme to help prevent contamination from amplified products.
- When used with Biori nucleic acid extraction or purification kits, the manual states a detection limit up to 5 CFU/mL.
- COA reports conforming negative concordance, positive concordance, LOD, and internal-standard Ct results.
Application
- Qualitative Mycoplasma DNA contamination testing.
- Testing of cell cultures, animal sera, and related biological materials.
- qPCR workflows using supported real-time PCR systems listed in the manual.
- Sample extraction workflows paired with a suitable nucleic acid extraction or purification kit.
Components
| Component | Specification |
|---|---|
| My qPCR MIX | 1.0 mL x 1 tube |
| My Positive Control | 1.5 mL x 2 tubes |
| My Negative Control | 1.5 mL x 2 tubes |
| My Internal Control | 1.0 mL x 1 tube |
| Instructions | 1 copy |
Shipping and Storage
| Condition | Recommendation |
|---|---|
| Storage | <= -20 C, protected from light. Shelf life: 24 months. Avoid more than 10 freeze-thaw cycles. |
| Shipping | Ship frozen on dry ice or with validated frozen cold-chain packaging that maintains the specified frozen storage condition. |
| Handling | Protect from light and avoid unnecessary freeze-thaw cycles where applicable. |
Data
COA lot 20250604 reports conforming negative concordance, positive concordance, LOD concordance, internal-standard Ct <= 35, and overall compliance with the enterprise standard.
