One-Step PAGE Gel Fast Preparation Kit (12.5%)

One-Step PAGE Gel Fast Preparation Kit (12.5%) is suitable for Tris-glycine electrophoresis systems and uses premixed formulations for stacking gel and separating gel.

One-step casting

No TEMED, odor-free

Sharp low-MW bands

Clearer than traditional gels

Product overview

One-Step PAGE Gel Fast Preparation Kit (12.5%) is suitable for Tris-glycine electrophoresis systems and uses premixed formulations for stacking gel and separating gel. Gel casting can be completed simply by adding Improved Coagulant, making the workflow convenient and fast. The prepared stacking gel is red, allowing the sample wells to be clearly distinguished for convenient loading. The red stacking gel can also be used to differentiate gels that contain different samples. This product is supplied with Improved Coagulant, which provides improved stability and catalytic efficiency, so no additional TEMED is required during gel preparation.

Features and applications

Features

Easy preparation: one-step gel casting, simple and fast
Colored stacking gel
Odor-free: no TEMED required, no unpleasant smell
Sharp bands: especially clearer small-molecular-weight protein bands than in traditional gels

Applications

Protein separation for Western blot / immunoblotting
Monitoring recombinant protein & antibody expression and purification
Protein purity analysis
Protein molecular weight determination
Verification of protein–protein interaction (Co-IP, pull-down) products

Components

Components	BR4C334-01 125 gels (0.75 mm)
Stacking Gel Solution (2×)	80 mL
Red Stacking Gel Buffer (2×)	80 mL
Separating Gel Solution 12.5% (2×)	250 mL
Separating Gel Buffer 12.5% (2×)	250 mL
Improved Coagulant	8 mL

Performance data

Schematic diagram of protein marker bands on resolving gels with different concentrations

Electrophoretic patterns of protein markers on resolving gels with different concentrations

BR4C334 One-Step PAGE Gel Fast Preparation Kit (12.5%): Electrophoretic patterns of protein markers on resolving gels with different concentrations

Conclusion: Across all five gel concentrations, the bands were sharp-edged with clean backgrounds and neat lanes, and each molecular weight band of the marker was well resolved — meeting the needs of full-range protein separation from high- to low-molecular-weight proteins. The kit offers five commonly used concentrations — 6%, 7.5%, 10%, 12.5% and 15% — allowing proteins of different molecular weights to find their ideal separation conditions.