Pyrophosphatase, Inorganic GMP-grade
Recombinant inorganic pyrophosphatase for IVT workflows.
Product overview
Pyrophosphatase, Inorganic is produced by recombinant expression in E. coli and purified. It catalyzes the hydrolysis of inorganic pyrophosphate to orthophosphate. During IVT, Pyrophosphatase, Inorganic hydrolyzes the inorganic pyrophosphate generated by the reaction, preventing inhibition of the reaction system and shifting the reaction equilibrium toward product formation. In molecular biology, it is used in IVT and can significantly increase RNA yield.
Available versions
| Cat. no. | Product name | Formulation |
|---|---|---|
| GMP-BP-E03 | Pyrophosphatase, Inorganic GMP-grade | |
| GMP-BP-E03-1K | Pyrophosphatase, Inorganic GMP-grade |
Features and applications
- Recombinant inorganic pyrophosphatase expressed and purified from E. coli; activity: 0.1 U/μL.
- Unit definition: one unit (U) is defined as the amount of enzyme required to catalyze the hydrolysis of inorganic pyrophosphate (PPi) to produce 1 μmol of inorganic phosphate (Pi) per minute.
- Quality control: protein purity ≥95%; free of exogenous DNase, RNase, exonuclease, and endonuclease activity; residual host-cell DNA ≤100 pg/mg; residual host-cell protein <50 ppm; bacterial endotoxin <5 EU/mL.
- Used to hydrolyze the inorganic pyrophosphate generated during IVT, thereby preventing inhibition by the inorganic pyrophosphate by-product, shifting the chemical reaction equilibrium forward, and increasing in vitro transcription yield.
Components
| Components | Cat. No. | Quantity | Volume |
|---|---|---|---|
| Pyrophosphatase, Inorganic GMP-grade (0.1 U/µL) | GMP-BP-E03-10 | 10 U | 100 µL |
| GMP-BP-E03-100 | 100 U | 1 mL | |
| GMP-BP-E03-1K | 1 KU | 10 mL |
Performance data
1 Purity analysis
Description: SDS-PAGE was used to analyze the purity of Pyrophosphatase, Inorganic.
Conclusion: The SDS-PAGE test results show that the Pyrophosphatase, Inorganic has high purity.
2 IVT test
Description: Compare the transcriptional yields in the same transcriptional system with and without the addition of Pyrophosphatase, Inorganic.
Conclusion: During IVT, adding a small amount of Pyrophosphatase, Inorganic significantly increases yield.
Shipping and storage
| Condition | Recommendation |
|---|---|
| Storage | Store at -20±5°C. |
| Shipping | Ship frozen on dry ice or with validated frozen cold-chain packaging that maintains the specified frozen storage condition. |
| Handling | Avoid repeated freeze-thaw cycles; mix thoroughly after thawing where applicable. |
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