RNase Inhibitor GMP-grade
Recombinant RNase inhibitor for protecting RNA workflows.
Product overview
RNase Inhibitor GMP-grade is a recombinant RNase inhibitor produced through recombinant expression and multi-step purification. It binds RNases with high affinity at a 1:1 ratio through noncovalent interactions, thereby inhibiting the activity of RNase A, RNase B, and RNase C without affecting the activity of RNase H or S1 Nuclease. This product does not inhibit phage RNA polymerases (SP6, T7, or T3), Taq, AMV, or MMLV. It also exhibits greater oxidation resistance and remains stable at lower DTT concentrations. It is suitable for preventing RNase contamination during IVT.
Available versions
| Cat. no. | Product name | Formulation |
|---|---|---|
| GMP-BP-E02-4K | RNase Inhibitor GMP-grade | |
| GMP-BP-E02-40K | RNase Inhibitor GMP-grade | |
| GMP-BP-E02-400K | RNase Inhibitor GMP-grade |
Features and applications
- (1) High purity
- (2) High activity
- (3) Strict residual control
- During IVT, it is used to inhibit RNases in the environment and system, thereby preventing degradation of the synthesized mRNA.
- mRNA storage and transport.
- mRNA isolation and purification.
- Reverse transcription and qPCR.
- Applications requiring intact RNA.
Components
| Components | Cat. No. | Quantity | Volume |
|---|---|---|---|
| RNase Inhibitor GMP-grade (40 U/μL) | GMP-BP-E02-4K | 4 KU | 100 μL |
| GMP-BP-E02-40K | 40 KU | 1 mL | |
| GMP-BP-E02-400K | 400 KU | 10 mL |
Performance data
1 Functional test
Description: RNA was used as the template, and qPCR was performed to verify the protective effect of RNase Inhibitor on the RNA template.
Conclusion: qPCR verification showed that adding RNase Inhibitor provided significant protection for RNA.
2 IVT test
Description: RNase Inhibitor was added to the IVT system, product integrity exceeded 95%.
Conclusion: Adding RNase Inhibitor during the IVT can result in a product with an integrity of over 95%.
Shipping and storage
| Condition | Recommendation |
|---|---|
| Storage | Store at -20±5°C. Mix thoroughly before use. Avoid repeated freeze-thaw cycles. |
| Shipping | Ship frozen on dry ice or with validated frozen cold-chain packaging that maintains the specified frozen storage condition. |
| Handling | Avoid repeated freeze-thaw cycles; mix thoroughly after thawing where applicable. |
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