RNase Residual Quantitative Detection Kit, BP-06

Quantifies residual RNase activity in mRNA workflows.

0.078 pg

RNase limit of detection

60 min

Fluorescence time course

R² > 0.99

Linear standard curve

RNase Residual Quantitative Detection Kit

Product overview

Ribonuclease (RNase) is a class of nucleases that catalyzes RNA degradation into small fragments. RNase A is a widely used endonuclease that specifically cleaves phosphodiester bonds on single-stranded RNA at the 3' end of cytidine (C) and uridine (U) residues, producing oligonucleotides with 2',3'-cyclic phosphate termini. Biori's RNase Residual Quantitative Detection Kit employs a fluorescent probe-based approach for rapid, high-sensitivity detection of RNase A activity. The kit utilizes a synthetic RNA oligonucleotide probe with a FAM fluorophore (donor) at one end and a TAMRA quencher (acceptor) at the other. When the two groups are in close proximity, fluorescence energy transfers from donor to acceptor, quenching the donor's fluorescence. Upon cleavage by RNase, the probe separates, the two groups disperse, and FAM fluorescence is no longer quenched, resulting in detectable fluorescence. The rate of fluorescence signal increase correlates positively with enzyme quantity and activity.

Features and applications

Features

(1) Based on enzymatic activity, designed for residual RNase detection.
(2) High sensitivity: LOD down to 0.078 pg RNase.
(3) Rigorous QC ensures sensitivity, inter/intra assay variation, accuracy.

Applications

(1) Biopharmaceutical and biological product QC: process residual detection and raw material/consumable monitoring;
(2) Molecular biology experiment support;
(3) Production and lab environment monitoring.

Components

Product Name	Cat. No.	Components	Volume
RNase Residual Quantitative Detection Kit	BP-AS-18	RNase A(10 mg/mL)	10 μL
	BP-AS-19	RNase Substrate	1 mL
	BP-AS-20	10× Reaction Buffer	1 mL
	BP-AS-11	RNase-free ddH₂O	1 mL×5

Performance data

1 Assay principle

Description: Schematic diagram of the Biori RNase Residual Quantitative Detection Kit.

BP-06-100 RNase Residual Quantitative Detection Kit: Figure 1 Schematic diagram of the Biori RNase Residual Quantitative Detection Kit — **Figure 1** Schematic diagram of the Biori RNase Residual Quantitative Detection Kit

Conclusion: The rate of fluorescence signal increase correlates positively with enzyme quantity and activity.

2 Sensitivity

Description: The sensitivity of the kit was evaluated by detecting varying amounts of RNase A.

Conclusion: The kit exhibits a sensitivity of 0.078 pg RNase.

3 Fluorescence intensity plot

Description: Time course of fluorescence intensity for the detection of various amounts of RNase A using this kit over a 60-minute period.

BP-06-100 RNase Residual Quantitative Detection Kit: Figure 3 Time course of fluorescence intensity for the detection of various amounts of RNase A using this kit over a 60-minute period — **Figure 3** Time course of fluorescence intensity for the detection of various amounts of RNase A using this kit over a 60-minute period

Conclusion: The figure shows the changes in fluorescence intensity of RNase A over a 60-minute period.

4 Standard curve

Description: Establishing the standard curve by gradient dilution of RNase A standard.

Conclusion: The RNase A standard were subjected to gradient dilution for fluorescence measurement. The data were processed using linear fitting. The results showed that the linear R² value was greater than 0.99.

Shipping and storage

Condition	Recommendation
Storage	Store at -20±5°C. Protect RNase Substrate (BP-AS-19) from light.
Shipping	Ship frozen on dry ice or with validated frozen cold-chain packaging that maintains the specified frozen storage condition.
Handling	Protect from light and avoid unnecessary freeze-thaw cycles where applicable.