DNase Residual Quantitative Detection Kit (Fluorescent Probe Method)

Quantifies residual DNase using a fluorescent-probe method.

1.25×10⁻⁵ U

DNase limit of detection

30 min

Fluorescence time course

R² > 0.99

Linear standard curve

Product overview

DNase Residual Quantitative Detection Kit (Fluorescent Probe Method), BP-16, is designed for quantitative detection of residual DNase in test samples using a fluorescent-probe method.

Features and applications

Features

(1) Based on enzymatic activity, designed for residual DNase detection.
(2) High sensitivity: LOD down to 1.25×10^-5 U DNase.
(3) Rigorous QC ensures sensitivity, inter/intra assay variation, accuracy.
For Research Use Only. Not for use in diagnostic procedures.

Applications

(1) Biopharmaceutical and biological product QC: process residual detection and raw material/consumable monitoring;
(2) Molecular biology experiment support;
(3) Production and lab environment monitoring.
For Research Use Only. Not for use in diagnostic procedures.

Components

Component	Pack size
DNase I (BP-E04)	10 µL
DNase Substrate (BP-AS-36)	0.8 mL
10× DNase I Reaction Buffer (BP-AS-13)	1 mL
DNase-free ddH2O (BP-AS-11)	10 mL

Performance data

1 Assay principle

Description: Schematic diagram of the Biori DNase Residual Quantitative Detection Kit (Fluorescent Probe Method).

BP-16 DNase Residual Quantitative Detection Kit (Fluorescent Probe Method): Figure 1 Schematic diagram of the Biori DNase Residual Quantitative Detection Kit (Fluorescent Probe Method). — **Figure 1** Schematic diagram of the Biori DNase Residual Quantitative Detection Kit (Fluorescent Probe Method).

Conclusion: The schematic illustrates the working principle of the DNase Residual Quantitative Detection Kit (Fluorescent Probe Method).

2 Fluorescence intensity plot

Description: Time course of fluorescence intensity for the detection of various amounts of DNase I using this kit over a 30 minutes period.

BP-16 DNase Residual Quantitative Detection Kit (Fluorescent Probe Method): Figure 2 Time course of fluorescence intensity for the detection of various amounts of DNase I using this kit over a 30 minutes period — **Figure 2** Time course of fluorescence intensity for the detection of various amounts of DNase I using this kit over a 30 minutes period

Conclusion: The figure shows the changes in fluorescence intensity of DNase I over a 30-minute period.

3 Standard curve

Description: Establishing the standard curve by gradient dilution of DNase I standard.

Conclusion: The DNase I standard were subjected to gradient dilution for absorbance measurement. The data were processed using a Linear fitting. The results showed that the linear R² value was greater than 0.99.

Shipping and storage

Condition	Recommendation
Storage	Store at -20±5°C. Protect DNase Substrate (BP-AS-36) from light.
Shipping	Ship frozen on dry ice or with validated frozen cold-chain packaging that maintains the specified frozen storage condition.
Handling	Protect from light and avoid unnecessary freeze-thaw cycles where applicable.