AcuGenix™ Neoscript 1st cDNA Synthesis Master Mix for qPCR (+ gDNA wiper)

All-in-one first-strand cDNA synthesis master mix with integrated gDNA removal, 50 °C reverse transcription and a ready-to-use format for two-step RT-qPCR.

50 °C RT

High-temperature reverse transcription

gDNA wiper

Removes 3.75 ng gDNA per reaction

All-in-one

Ready-to-use premix

AcuGenix™ Neoscript 1st cDNA Synthesis Master Mix for qPCR (+ gDNA wiper), BR1D301 product image

Product overview

AcuGenix™ Neoscript 1st cDNA Synthesis Master Mix for qPCR (+ gDNA wiper) contains all reagents required for first-strand cDNA synthesis and is suitable for two-step RT-qPCR detection. 4×gDNA Wiper Mix can thoroughly eliminate residual genomic DNA contamination in RNA templates, ensuring more accurate quantification results. It also simplifies qPCR primer design, eliminating the need for intron-spanning primers. The 5×AcuGenix™ Neoscript 1st cDNA Synthesis Master Mix for qPCR is an efficient and convenient first-strand cDNA synthesis master mix. The reverse transcription reaction can be performed simply by adding the RNA template and nuclease-free water.

cDNA synthesized with this product is compatible with both fluorescent dye and probe-based qPCR platforms. Corresponding supporting reagents can be selected according to experimental purposes for high-performance gene expression analysis.

Features and applications

Features

Optimized for 50°C high-temperature reverse transcription
Ready-to-use all-in-one premix
Integrated genomic DNA removal module.

Applications

Reverse transcription (with genomic DNA removal)
Gene expression analysis
Two-step RT-qPCR

Components

Components	BR1D301-01 100 T (20 μL/T)	BR1D301-02 500 T (20 μL/T)
4×gDNA Wiper Mix	40 µL	400 µL
5×AcuGenix™ Neoscript 1st cDNA Synthesis Master Mix for qPCR	40 µL	400 µL
5×No RT Control Mix	4 µL	40 µL
RNase-free ddH₂O	200 µL	1 mL

Performance data

gDNA Removal Assay

Human genomic DNA was digested with gDNA Wiper Mix sourced from various suppliers and Biori, followed by inactivation treatment. The digested human genomic DNA was subjected to qPCR analysis using AcuGenix™ Superstart All-in-One qPCR Master Mix with UNG (Probe qPCR, Cat.: M2071).

Conclusions: BR1D301 fully eliminates 3.75 ng human genomic DNA per reaction, matching the performance of Supplier A and Supplier B.

Reverse Transcription Efficiency

Total human RNA was treated with cDNA synthesis kits from Biori, Supplier A and B. The resulting cDNA was subjected to qPCR assay with AcuGenix™ Robustart qPCR Master Mix (Universal ROX) (Cat.: BR1E101).