AcuGenix™ Neoscript 1st cDNA Synthesis Kit (+gDNA wiper)

This kit contains all reagents required for first-strand cDNA synthesis, and is widely applicable to nucleic acid detection assays such as RT-qPCR and conventional PCR.

5 min

Reverse transcription

Up to 10 kb

cDNA length

10 pg RNA

Specific bands at low input

AcuGenix™ Neoscript 1st cDNA Synthesis Kit (+gDNA wiper), BR1D201 product image

Product overview

This kit contains all reagents required for first-strand cDNA synthesis, and is widely applicable to nucleic acid detection assays such as RT-qPCR and conventional PCR. The included 4×Neoscript RT Core Mix is a highly efficient premix for rapid first-strand cDNA synthesis with an optimized formulation. In operation, users only need to add appropriate reverse transcription primers according to the requirements of downstream applications. The 4×gDNA Wiper Mix component can efficiently and thoroughly remove residual genomic DNA (gDNA) from RNA templates, effectively eliminating gDNA interference during PCR amplification. cDNA products synthesized with this kit exhibit excellent compatibility and are adaptable to a variety of PCR detection formats. Laboratory personnel may select corresponding supporting reagents for combined use based on specific experimental purposes and detection requirements.

Features and applications

Features

High sensitivity
5 min fast reverse transcription
Ultra-high thermal stability
Synthesizes cDNA up to 10 kb in length

Applications

Reverse transcription (with genomic DNA removal)
Gene expression analysis
Two-step RT-qPCR

Components

Component	BR1D201-01 100 T (20 μL/T)	BR1D201-02 500 T (20 μL/T)
4×gDNA Wiper Mix	300 µL	1.5 mL
4×Neoscript RT Core Mix	500 µL	2.5 mL
Oligo (dT)₁₈ VN	100 µL	500 µL
Random 6 mers	100 µL	500 µL
4×No RT Control Mix	50 µL	250 µL
RNase-free ddH₂O	2 mL	10 mL

Performance data

High sensitivity

Total RNA extracted from HEK-293T cells at different concentrations was used as templates. Reverse-transcription reactions were performed using Biori BR1D201 and a commercial comparable reverse-transcription reagent (Supplier A), respectively. The resulting cDNA products were amplified by PCR with Biori BR1A401.

Conclusion: At identical RNA template amounts, BR1D201 exhibited higher yields. Distinct specific amplification bands can still be observed even at low RNA template inputs of 100 pg and 10 pg, demonstrating significantly better performance than the comparable product (Supplier A).

5 min fast reverse transcription

Total RNA of HEK-293T cells at 100 ng was used as the template. Reverse-transcription reactions were carried out with Biori BR1D201 and a commercial comparable reverse transcription product, with reaction durations set at 5 min, 15 min, 30 min and 45 min. The resulting cDNA products were subjected to PCR amplification using Biori BR1A401.

Conclusion: BR1D201 achieves efficient reverse-transcription in merely 5 min, and its amplification performance is largely consistent with that obtained with extended reaction durations.

Ultra-high thermal stability

Using 100 ng total RNA from HEK-293T cells as template, reverse-transcription reactions were performed with Biori BR1D201 and a commercial comparable reverse transcription product under gradient temperatures of 60 °C, 58 °C, 56.7 °C, 53 °C, 49 °C and 46 °C. The resulting cDNA products were amplified by PCR using Biori BR1A401.

Conclusion: As the reaction temperature increased, the reverse-transcription efficiency of the commercial product decreased significantly at 56.7 °C and continued to decline. In contrast, BR1D201 maintained excellent reverse transcription activity even at a high temperature of 60 °C.

Synthesizes cDNA up to 10 kb in length

Using 100 ng total RNA from HEK-293T cells as template, reverse transcription reactions were performed with Biori BR1D201 and a commercial comparable reverse transcription product under identical reaction systems. The resulting cDNA products were amplified for target fragments of different lengths using Biori BR1A401.

Conclusion: Compared with the competitor, BR1D201 yields distinct bands when amplifying long fragments of approximately 10 kb, indicating its markedly superior performance in long-fragment reverse transcription relative to the comparable product.