AcuGenix™ RNase Inhibitor (40 U/μL)
RNase inhibitor for protecting RNA-sensitive reactions.
Product overview
This product is a recombinant mouse RNase inhibitor expressed and purified in a soluble form in E. coli, capable of broadly inhibiting various types of RNases (RNase A, B, C). The principle of its specific inactivation of RNase is to bind non-covalently with RNase to form a complex, causing RNase to become inactive, thus protecting RNA from degradation. This product is compatible with various commercial Reverse Transcriptase, DNA Polymerases, SP6, T7, or T3 RNA polymerases and AMV. Compared with human RNase inhibitor, murine RNase inhibitor lacks two cysteines that are very sensitive to oxidation in human protein, so it has higher antioxidant activity and is more suitable for experiments sensitive to high DTT (such as RT-PCR).
Features and applications
- Capable of broadly inhibiting various RNases (RNase A, B, C).
- Enhanced oxidative resistance.
- cDNA synthesis
- RT-PCR / qPCR / PCR
- RNA isolation and purification
Components
| Components | BR1D901-01 2,000 U | BR1D901-02 10,000 U | BR1D901-03 20,000 U |
|---|---|---|---|
| AcuGenix™ RNase Inhibitor (40 U/μL) | 0.05 mL | 0.25 mL | 0.5 mL |
Performance data
RNase inhibition test
RNase at a series of concentrations was mixed with an equal volume of RNase inhibitor from different suppliers and then incubated with a human total RNA template. After treatment, the RNA samples were amplified by RT-qPCR. The results are shown below:
Conclusion: Biori BR1D901 exhibits higher RNase A inhibitory activity than comparable products from Suppliers A, B, and C.
Shipping and storage
| Condition | Recommendation |
|---|---|
| Storage | Store at -20±5°C |
| Shipping | Refer to product label and shipping documentation. |
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