AcuGenix™ AmpHiFi Mul-RT-PCR Kit

Closed-Tube, One-Workflow Solution for High-Fidelity, Ultra-Multiplex RNA Target Amplification

10 copies

Limit of detection for viral RNA

400+ plex

Multiplex panels

10 s / kb

Extension rate, 25–85% GC

AcuGenix™ AmpHiFi Mul-RT-PCR Kit, BR3M322 product image

Product overview

The AcuGenix™ AmpHiFi Mul-RT-PCR Kit is a premium closed-tube, one-workflow solution optimized for broad-spectrum RNA template amplification. It integrates reverse transcription (RT) and high-fidelity PCR into a single reaction via a synergistically engineered enzyme cocktail (derived from directed evolution) and a pre-optimized multiplex-compatible buffer system. The kit eliminates the need for intermediate purification or tube-opening steps, significantly minimizing aerosol contamination risks for high-throughput and clinical research workflows.

Features and applications

Features

Industry-Leading Performance Benchmarks: Rigorously batch-validated to deliver best-in-class metrics: a robust limit of detection (LoD) of 10 copies for target viral RNA, stable amplification of 400+plex multiplex panels, an ultra-fast extension rate of 10 sec/kb, and broad GC tolerance spanning 25–85% without requiring additional buffer optimization.
Engineered Enzyme Synergy: The thermostable reverse transcriptase delivers exceptional processivity and efficient RNA secondary structure unwinding capability, outperforming conventional RT enzymes with GC-rich and structurally complex RNA templates. It is paired with an antibody-blocked hot-start high-fidelity DNA polymerase that retains 3′→5′ exonuclease proofreading activity, offering ~68-fold higher fidelity than wild-type Taq DNA polymerase to minimize sequence errors.
Simplified, Contamination-Safe Workflow: Pre-formulated components require no intermediate handling, reducing pipetting errors and aerosol contamination risks associated with open-tube operations.
Optimized Multiplex Performance: The pre-optimized reaction buffer is supplemented with multiplex amplification enhancers and background suppression additives to effectively inhibit primer-dimer formation and non-specific amplification, enabling uniform amplification of multiplex targets without laborious primer titration.
Ultra-High Purity: Each batch is strictly quality-controlled to be free of detectable RNase, DNase, and extraneous nucleic acid contamination, ensuring reliable performance for low-abundance target detection.

Applications

Multiplex respiratory pathogen panels for simultaneous detection of RNA viruses and atypical pathogens
RNA virus variant screening and low-frequency mutation detection
Immune repertoire (TCR/BCR) analysis
Target enrichment for single-cell RNA-seq (scRNA-seq) and spatial transcriptomics workflows
Rare RNA target detection in liquid biopsy and minimal residual disease (MRD) research

Components

Component	Specification/Notes
2×RT PCR Buffer	/
RT-PCR Enzyme Mix	/

Performance data

Efficient amplification

BR3M322 AcuGenix™ AmpHiFi Mul-RT-PCR Kit: Figure 1 Comparative evaluation of multiplex RT-PCR amplification efficiency — **Figure 1 Comparative evaluation of multiplex RT-PCR amplification efficiency.** The amplification efficiency of each target in the BR3M322 (AcuGenix™ AmpHiFi Mul-RT-PCR Kit) multiplex assay was highly consistent with that of single-target detection workflows.

Note: BR3M322 is the catalog number for this kit. Consistent amplification efficiency between multiplex and single-target workflows is a critical performance metric for ultra-multiplex assays, ensuring balanced representation of all targets and minimizing detection bias.