AcuGenix™ Mul-PCR Mix for DNA&RNA

Single-Tube RT-Multiplex Master Mix for Simultaneous DNA & RNA Target Detection

DNA + RNA

Single-tube RT-multiplex PCR

400+ plex

Validated with a tNGS panel, low primer dimer

Ready-to-use

Add template, primers and water

AcuGenix™ Mul-PCR Mix for DNA&RNA, BR3M303 product image

Product overview

The AcuGenix™ Mul-PCR Mix for DNA&RNA is a ready-to-use multiplex master mix optimized for high-throughput, simultaneous detection of diverse nucleic acid templates. It innovatively integrates reverse transcription (RT) and multiplex PCR into a single-tube reaction system, powered by a proprietary buffer formulation and a high-performance engineered enzyme blend. All core reaction components-including a chemically modified hot-start DNA polymerase, a directed evolution-derived thermostable reverse transcriptase, optimally formulated Mg²⁺, dNTPs, and multiplex amplification enhancers-are pre-formulated for immediate use, eliminating the need for additional supplementation of any core reagents.

Features and applications

Features

Single-Tube RT-Multiplex Workflow: Consolidates RNA reverse transcription and multiplex PCR into one closed-tube reaction, removing the need for multi-step tube transfers, drastically streamlining workflows while minimizing pipetting errors and aerosol contamination risks.
Broad Template Compatibility: Overcomes the DNA-only limitation of conventional multiplex master mixes, supporting simultaneous amplification of both DNA and RNA targets without buffer re-optimization.
Ready-to-Use Convenience: Users only need to add DNA and/or RNA templates, multiplex gene-specific primers, and nuclease-free water to initiate the full workflow spanning RT, PCR amplification, and direct DNA target amplification.
Ultra-High Purity: Each batch is rigorously quality-controlled to be free of detectable RNase, DNase, and extraneous nucleic acid contamination, ensuring reliable performance for low-abundance target detection.
Reproducible Performance: Stringent batch-to-batch consistency validation guarantees stable amplification results across all production lots.

Applications

Multiplex respiratory pathogen panels for simultaneous detection of DNA and RNA viruses

Components

Component	Specification/Notes
AcuGenix™ Mul-PCR Mix for DNA&RNA	/

Performance data

Low Primer Dimer Formation

BR3M303 AcuGenix™ Mul-PCR Mix for DNA&RNA: Figure 1 Performance validation in ultra-multiplex RT-PCR workflows — **Figure 1 Performance validation in ultra-multiplex RT-PCR workflows.** BR3M303 (AcuGenix™ Mul-PCR Mix for DNA&RNA) was evaluated in combination with a 400+ plex tNGS (targeted Next-Generation Sequencing) panel for amplification of nucleic acids extracted from clinical specimens, enterprise reference materials, and control samples. Amplified products exhibited high specificity with no obvious primer dimers.

Note: Primer dimers are a common byproduct of multiplex PCR that consume sequencing reads and reduce usable data yield. Minimal primer dimer formation preserves sequencing capacity for target amplicons, improving pathogen detection sensitivity and data utilization efficiency in tNGS workflows. BR3M303 is the catalog number for this product.