Bst DNA Polymerase · Cat. no. E103
AcuGenix™ Bst Hot Start DNA Polymerase-V2
AcuGenix™ Bst Hot Start DNA Polymerase-V2, E103, is supplied for DNA strand-displacement reactions.
No activation step
Aptamer hot start, room-temperature setup
GC-rich & ng templates
Sequencing-grade LAMP performance
Product overview
AcuGenix™ Bst Hot Start DNA Polymerase-V2 is derived from Bacillus stearothermophilus DNA Polymerase I. AcuGenix™ Bst Hot Start DNA Polymerase-V2 retains the activity of 5'→3' DNA polymerase by genetic engineering technology, removes the activity of 5'→3' exonuclease, and has the ability of strand displacement.
Available versions
Features and applications
Features
- Aptamer-modified hot-start isothermal amplification enzyme; no separate activation step is required.
- Reactions can be assembled at room temperature to help limit nonspecific amplification.
- Designed for LAMP.
Applications
- DNA strand-displacement reactions.
- LAMP isothermal amplification.
- DNA sequencing of GC-rich templates and nanogram-level templates.
Components
| Component | Specification / Notes |
|---|---|
| 8 U/µL AcuGenix™ Bst Hot Start DNA Polymerase-V2 | 8U/μL |
| 10×LAMP Buffer (Mg2+ free) (optional) | Included component; refer to the instruction manual |
| 35.71× MgSO4 (optional) | Included component; refer to the instruction manual |
Shipping and storage
| Condition | Recommendation |
|---|---|
| Shipping Temperature | Ice pack transportation (2-8°C) |
| Storage Temperature | -20±5°C |
| Shelf Life | 24M |
Downloads
AcuGenix™ Bst Hot Start DNA Polymerase-V2 (Glycerol-Free) (FE103)
Instruction Manual (PDF)COA (PDF)MSDS (PDF)
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