AcuGenix™ Bst Hot Start DNA Polymerase-V3

1. High/Low Template Balance (Amplification Efficiency)

■NTC ■0.0125 ng/μl ■0.025ng/μl ■0.125 ng/μl ■0.25ng/μl ■1.25 ng/μl
Channel	Amplification Curve	Raw Curve
E105

Ct Value Statistics

Conclusion 1: At each of the concentrations 0.0125 ng/μl, 0.025ng/μl, 0.125 ng/μl, 0.25ng/μl, and 1.25 ng/μl, the CT value difference was < 0.5 min. E105 showed good template balance across the 5 concentrations.

2. Freeze-Thaw Stability Test

Stored at -20°C5 Freeze-Thaw Cycles10 Freeze-Thaw Cycles15 Freeze-Thaw Cycles20 Freeze-Thaw Cycles
Channel	0.1pg/μl	0.01pg/μl
FAM

Conclusion 1: After 5, 10, 15, and 20 freeze-thaw cycles, E105 showed stable amplification at 0.1 pg/μl and 0.01 pg/μl, with ΔCt < 2 min compared with the -20°C control.

3. Accelerated Stability Test

Stored at -20°C 37°C, 3 Days 37°C, 7 Days 37°C, 14 Days
Channel	0.1pg/μl	0.01pg/μl
FAM

Conclusion 1: After accelerated aging at 37°C for 7 days and at 37°C for 14 days, E105 showed stable amplification at 0.1 pg/μl and 0.01 pg/μl, with ΔCt < 2 min compared with the -20°C control.

4. Enzyme Activity Assay

Conclusion 1: E105 effectively remained inactive at 30°C and was fully activated at 65°C.