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Product Catalog
AcuGenix™ RNase H II is a thermostable riboendonuclease that specifically cleaves the phosphodiester bond at the 5′ side of a ribonucleotide within a DNA/RNA heteroduplex, generating a 3′-hydroxyl terminus and a 5′-phosphate group. Unlike RNase H1, this enzyme requires a minimum of one ribonucleotide embedded within a DNA strand for activity, and does not cleave single-stranded or double-stranded RNA or DNA.
Features
- Designed for LAMP probe-based assays,facilitating hydrolysis probe cleavage.
- Specifically recognizes ribonucleotide bases within DNA duplexes, cleaving the phosphodiester bond between the RNA base (5'direction) and the adjacent DNA base,generating a 3 '-hydroxyl terminus and a 5 '-phosphate group on the ribonucleotide.
Application
- Molecular diagnostic research and manufacturing workflows.
Components
| Component | Specification / Notes |
|---|---|
| RNase H II (200 mU/μL) | 200 mU/μL |
| Bst2.0 HS (8 U/µL) (Sold separately) | Included component; refer to the instruction manual |
| LAMP Master Mix Buffer II (Sold separately) | Included component; refer to the instruction manual |
Shipping and Storage
| Condition | Recommendation |
|---|---|
| Shipping Temperature | Ice pack transportation（2-8℃） |
| Storage Temperature | -20±5℃ |
| Shelf Life | 24M |
Data
Coming soon.
