Nuclease · Cat. no. E201
AcuGenix™ RNase H II
AcuGenix™ RNase H II, E201, is supplied for molecular diagnostic research and manufacturing workflows.
Probe cleavage
For LAMP hydrolysis-probe assays
3′-OH / 5′-P
Defined cleavage ends at the ribonucleotide
Product overview
AcuGenix™ RNase H II is a thermostable riboendonuclease that specifically cleaves the phosphodiester bond at the 5′ side of a ribonucleotide within a DNA/RNA heteroduplex, generating a 3′-hydroxyl terminus and a 5′-phosphate group. Unlike RNase H1, this enzyme requires a minimum of one ribonucleotide embedded within a DNA strand for activity, and does not cleave single-stranded or double-stranded RNA or DNA.
Available versions
Features and applications
Features
- Designed for LAMP probe-based assays, facilitating hydrolysis probe cleavage.
- Specifically recognizes ribonucleotide bases within DNA duplexes, cleaving the phosphodiester bond between the RNA base (5' direction) and the adjacent DNA base, generating a 3'-hydroxyl terminus and a 5'-phosphate group on the ribonucleotide.
Applications
- Molecular diagnostic research and manufacturing workflows.
Components
| Component | Specification / Notes |
|---|---|
| RNase H II (200 mU/μL) | 200 mU/μL |
| Bst2.0 HS (8 U/µL) (Sold separately) | Included component; refer to the instruction manual |
| LAMP Master Mix Buffer II (Sold separately) | Included component; refer to the instruction manual |
Shipping and storage
| Condition | Recommendation |
|---|---|
| Shipping Temperature | Ice pack transportation (2-8°C) |
| Storage Temperature | -20±5°C |
| Shelf Life | 24M |
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