AcuGenix™ HandyAmp Fast PCR Master Mix (Dye plus)
Ready-to-use 2× fast PCR premix with dye-plus format.
Product overview
AcuGenix™ HandyAmp Fast PCR Master Mix is a product containing a genetically engineered hot-start DNA polymerase with a high amplification success rate. This polymerase possesses extremely high DNA binding affinity and strong processivity, enabling rapid amplification with an extension rate of 1–30 s/kb (≤2 kb: 1 s/kb, ≤5 kb: 5 s/kb, ≤10 kb: 10 s/kb). It features broad template compatibility, suitable for genomic DNA or crude templates from animals, plants and bacteria, as well as target sequences with high or low GC content. In addition, this product is pre-formulated as a 2×Premix. Amplification can be performed simply by adding primers and template. This Dye plus version is pre-loaded with electrophoresis loading dye, allowing direct electrophoresis of PCR products for convenient and efficient operation.
Features and applications
- Rapid amplification: 1–30 s/kb (≤2 kb: 1 s/kb; ≤5 kb: 5 s/kb; ≤10 kb: 10 s/kb);
- High success rate: Compatible with crude genomic extracts from animals/bacteria & GC-variable targets;
- Pentaplex amplification capability;
- Convenient workflow: Direct gel electrophoresis without extra dye addition.
- Rapid PCR
- Genotyping
- Colony PCR
Components
| Component | BR1A401-11 | BR1A401-12 | BR1A401-13 |
|---|---|---|---|
| AcuGenix™ HandyAmp Fast PCR Master Mix (Dye plus) | 5×1 mL | 25×1 mL | 50×1 mL |
Performance data
Rapid Amplification
Conclusion: Biori BR1A401 only needs 1 hour to complete the amplification of DNA fragments within 5 kb, showing rapid amplification performance.
Rapid Amplification of DNA Fragments within 2 kb (Genomic DNA or Crude Templates)
1: λDNA 0.9 kb
2: E. coli colony 0.9 kb
3: Yeast colony 1 kb
4: Rice lysates 1.5 kb
5: Mouse gDNA 2 kb
6: Human gDNA 1 kb
Conclusion: Using different types of templates, Biori BR1A401 can complete the amplification of DNA fragments within 2 kb at an extension rate of 1 s/kb.
Rapid Amplification of Fragments within 10 kb
Conclusion: The extension rate of Biori BR1A401 for fragments within 10 kb can reach 10 s/kb.
Compatibility with GC-poor/rich systems
M: DL2000; 1: human gDNA - 30% GC; 2: human gDNA - 61% GC; 3: human gDNA - 81% GC.
Conclusion: Biori BR1A401 is capable of amplifying target DNA of high- and low-GC content.
Multiplex Amplification
Conclusion: Biori BR1A401 can complete 5-plex amplification, and multiple targets can be detected in the same reaction.
Shipping and storage
| Condition | Recommendation |
|---|---|
| Storage | Store at -20±5°C |
| Shipping | Refer to product label and shipping documentation. |
Downloads
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