AcuGenix™ LongR Super-Fidelity Master Mix

2× Ready-to-Use High-Fidelity PCR Premix for Ultra-Long Fragment Amplification

~154× fidelity

Compared with wild-type Taq

Up to 30 kb

20 kb from genomic DNA

Crude templates

Whole blood, maize and yeast direct PCR

Product overview

The AcuGenix™ LongR Super-Fidelity Master Mix is a 2× ready-to-use high-fidelity PCR premix formulated with a genetically engineered hot-start high-fidelity DNA polymerase. The enzyme exhibits exceptional DNA binding affinity and processivity, delivering excellent compatibility with complex templates and partially degraded nucleic acids. With a fidelity approximately 154-fold higher than that of wild-type Taq DNA polymerase, the mix is optimized for high-accuracy long-range PCR workflows.

Proprietary extension enhancers and specificity-boosting additives are integrated into the formulation to significantly improve long-fragment amplification capability, reaction specificity, and yield. For simple templates such as λDNA or plasmids, the mix efficiently amplifies targets up to 30 kb; for complex templates such as genomic DNA, it reliably amplifies fragments up to 20 kb. The mix also demonstrates robust tolerance to common PCR inhibitors, enabling direct PCR from crude samples including bacteria, fungi, plant tissues, and whole blood without prior nucleic acid extraction.

All core components—including the hot-start high-fidelity DNA polymerase, dNTPs, and optimized buffer system—are pre-formulated; users only need to add primers and template to initiate amplification, reducing pipetting steps, minimizing human error, and improving assay reproducibility. A version supplemented with electrophoresis tracking dyes is also available, allowing direct gel loading post-PCR without additional loading buffer.

Features and applications

Features

Ultra-High Fidelity: ~154× higher fidelity than wild-type Taq polymerase, drastically reducing sequence errors for downstream cloning and sequencing applications.
Best-in-Class Long-Range Capability: Amplifies up to 30 kb from simple templates (λDNA, plasmids) and up to 20 kb from complex genomic DNA, outperforming conventional high-fidelity PCR mixes.
Exceptional Template Compatibility: Efficiently amplifies complex, partially degraded, or inhibitor-laden templates, eliminating the need for time-consuming sample purification for crude specimens.
Streamlined Workflow: 2× ready-to-use format reduces pipetting steps and improves reproducibility; optional tracking dye version enables direct gel loading for faster result validation.
Superior Specificity: Proprietary specificity enhancers minimize non-specific amplification and primer-dimer formation, ensuring clean amplification products.

Applications

Gene cluster cloning and BAC library construction
Ultra-long fragment assembly for genome finishing and structural variant analysis
Direct PCR from crude samples (bacteria, fungi, plant tissues, whole blood)
Long-range PCR for mutation screening and haplotype analysis
Template preparation for long-read sequencing (PacBio, Oxford Nanopore)

Components

Component	Specification/Notes
AcuGenix™ LongR Super-Fidelity Master Mix	1 mL / 5×1 mL / 15×1 mL

Performance data

Extension Rate Assay

BR3M121 AcuGenix™ LongR Super-Fidelity Master Mix: Figure 1. Extension rate evaluation for 1.5 kb–10 kb amplicons — **Figure 1. Extension rate evaluation for 1.5 kb–10 kb amplicons.** BR3M121 (AcuGenix™ LongR Super-Fidelity Master Mix) exhibits an extension rate of 1 sec/kb for templates <4 kb, and up to 5 sec/kb for 4–10 kb DNA fragments, significantly reducing long-range PCR cycling time and improving assay throughput.

Note: Rapid extension kinetics is a critical performance metric for long-range PCR, minimizing non-specific amplification and preserving amplicon integrity.

Template Compatibility Test

BR3M121 AcuGenix™ LongR Super-Fidelity Master Mix: Figure 2. Amplification performance across human gDNA, λDNA, plasmid, and cDNA templates — **Figure 2. Amplification performance across human gDNA, λDNA, plasmid, and cDNA templates.** BR3M121 delivers robust amplification across both simple (λDNA, plasmids) and complex (human genomic DNA, cDNA) templates, supporting workflows ranging from routine cloning to long-read sequencing library preparation.

Sensitivity Test

BR3M121 AcuGenix™ LongR Super-Fidelity Master Mix: Figure 3. Sensitivity benchmarking (1 pg–50 pg input) vs. competitors — **Figure 3. Sensitivity benchmarking (1 pg–50 pg input) vs. competitors.** BR3M121 demonstrated sensitivity identical to Supplier A, with reliable amplification down to 1 pg input, making it suitable for low-input and low-abundance target applications.

Note: 1 pg of human genomic DNA corresponds to ~150 genomic copies, supporting long-range amplification from trace sample inputs.

Crude Nucleic Acid Matrix Tolerance

BR3M121 AcuGenix™ LongR Super-Fidelity Master Mix: Figure 4. Performance on crude extracts (whole blood, maize, yeast) vs. competitors — **Figure 4. Performance on crude extracts (whole blood, maize, yeast) vs. competitors.** BR3M121 maintains high amplification efficiency and superior specificity when applied to unpurified crude extracts from plant tissues, bacteria, and whole blood, eliminating the need for time-consuming nucleic acid extraction.

BR3M121 AcuGenix™ LongR Super-Fidelity Master Mix: Figure 4 (panel 2) performance on crude extracts vs. competitors — **Figure 4. Performance on crude extracts (whole blood, maize, yeast) vs. competitors.** BR3M121 maintains high amplification efficiency and superior specificity when applied to unpurified crude extracts from plant tissues, bacteria, and whole blood, eliminating the need for time-consuming nucleic acid extraction.

GC Content Compatibility Test

BR3M121 AcuGenix™ LongR Super-Fidelity Master Mix: Figure 6. Performance on 25%–67% GC targets vs. competitors — **Figure 6. Performance on 25%–67% GC targets vs. competitors.** BR3M121 reliably amplifies targets with GC content ranging from 25% to 67%, delivering overall higher amplification efficiency and specificity than competing products.

Note: The formulation is optimized for a broad GC tolerance range of 25%–85% without requiring buffer re-optimization, accommodating the vast majority of GC-challenged templates.