Taq DNA Polymerase · Cat. no. E03
AcuGenix™ Hot Start Taq DNA Polymerase
AcuGenix™ Hot Start Taq DNA Polymerase, E03, is a glycerol-containing, chemically modified enzyme for specific and sensitive PCR and qPCR amplification.
Chemical hot start
Blocked at room temperature, time-release activation
Multiplex & low copy
Strong fluorescence signal in qPCR
Product overview
AcuGenix™ Hot Start Taq DNA Polymerase, E03, is a glycerol-containing, chemically modified enzyme for specific and sensitive PCR and qPCR amplification.
Available versions
| Cat. no. | Product name | Formulation | Residual DNA level |
|---|---|---|---|
| E03 | AcuGenix™ Hot Start Taq DNA Polymerase | Liquid (with glycerol) | Standard |
| FE03 | AcuGenix™ Hot Start Taq DNA Polymerase (Glycerol-Free) | Glycerol-free | Standard |
| E03-NA | AcuGenix™ Hot Start Taq DNA Polymerase-Low DNA | Liquid (with glycerol) | Low Residual DNA |
| FE03-NA | AcuGenix™ Hot Start Taq DNA Polymerase-Low DNA (Glycerol-Free) | Glycerol-free | Low Residual DNA |
Features and applications
Features
- Chemically modified hot-start enzyme with activity blocked at room temperature.
- Time-release activation during PCR temperature ramping supports specific amplification.
- Helps suppress non-specific amplification caused by primer mis-annealing and primer dimers.
- Designed for high sensitivity, strong fluorescence signals, and high specificity in real-time PCR.
- Suitable for multiplex PCR and amplification of low-copy templates.
- Provides 5'-3' polymerase and 5'-3' exonuclease activities; lacks 3'-5' proofreading activity.
- Generates PCR products with a 3' A overhang for direct T-vector cloning.
Applications
- Conventional PCR and endpoint PCR.
- Real-time PCR (qPCR) and qRT-PCR workflows after reverse transcription.
- Multiplex PCR and low-copy template amplification.
- Probe-based and fluorescent dye-based assays.
- Gene chip assays and other molecular detection workflows requiring hot-start amplification.
Components
| Component | Specification / Notes |
|---|---|
| AcuGenix™ Hot Start Taq DNA Polymerase | 5 U/µL |
| 10× PCR Buffer II (Mg²⁺ free) (optional) | Does not contain dNTPs or Mg²⁺. |
| MgCl2 (optional) | 25 mM |
Shipping and storage
| Condition | Recommendation |
|---|---|
| Shipping Temperature | Cold-chain shipment under conditions compatible with the stated frozen storage requirement. |
| Storage Temperature | Store at -20 ± 5°C. Vortex before use and avoid repeated freeze-thaw cycles. |
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