AcuGenix™ NGS DNA Clean Beads

Superparamagnetic Beads for Standardized, High-Recovery Nucleic Acid Purification & Size Selection

> 85% recovery

Top-tier NGS cleanup performance

AMPure XP-like

Identical protocol, 0.6×–1.8× ratios

Manual + automated

Compatible with all major NGS kits

Product overview

AcuGenix™ NGS DNA Clean Beads leverage a high-performance superparamagnetic bead matrix coupled with an optimized buffer system to enable efficient, reproducible purification and size selection of nucleic acid fragments. At defined bead-to-sample ratios, the beads selectively bind target fragments, allowing precise recovery of user-defined size ranges. The kit follows the same validated operating procedure as the industry gold-standard AMPure XP Beads, making it a seamless drop-in replacement for existing NGS workflows. It supports both manual experimental operation and high-throughput workflows on all major automated liquid-handling workstations.

Features and applications

Features

Precise Size-Selective Recovery: Adjustable fragment selection via bead-to-sample ratio tuning, enabling efficient recovery of target nucleic acid size ranges for diverse NGS applications.
Industry-Standard Workflow Compatibility: Identical operating procedure to AMPure XP Beads, requiring no protocol re-optimization for existing workflows.
Broad Kit Compatibility: Fully compatible with DNA and RNA library preparation kits from all major NGS vendors.
Manual & Automated Adaptability: Supports both manual benchtop operation and high-throughput workflows on leading automated liquid handling platforms, accommodating varying throughput requirements.
High Reproducibility: Batch-to-batch consistency ensures reliable, uniform purification results across experiments.

Applications

Post-step purification for NGS library construction (post-fragmentation, post-ligation, post-amplification)
Size selection for whole-genome sequencing (WGS), whole-exon sequencing (WES), RNA-seq, and targeted sequencing libraries
Cleanup of PCR products, enzymatic reaction products, and low-input nucleic acid samples

Components

Component	Specification/Notes
AcuGenix™ NGS DNA Clean Beads	1 mL / 5 mL / 60 mL / 450 mL

Performance data

High purification recovery efficiency

BR3N401 AcuGenix™ NGS DNA Clean Beads: Figure 1. Nucleic acid recovery performance of BR3N401 magnetic beads — **Figure 1. Nucleic acid recovery performance of BR3N401 magnetic beads.** The same amplified DNA substrate was purified using BR3N401 or four commercial bead sets (A–D). BR3N401 achieved >85% recovery, ranking among the top tier of domestic and international reagents. These results confirm high and consistent purification efficiency suitable for both routine and high-throughput library workflows.

Note: BR3N401 is the catalog number for AcuGenix™ NGS DNA Clean Beads. >85% recovery is a premium industry benchmark for NGS library purification, minimizing nucleic acid loss for low-input samples.

Bead Ratio and Input Flexibility

BR3N401 AcuGenix™ NGS DNA Clean Beads: Figure 2. Higher recovery with equivalent reproducibility to AMPure XP — **Figure 2. Higher recovery with equivalent reproducibility to AMPure XP.** BR3N401 was benchmarked against a commercial XP bead across a wide range of bead ratios and DNA inputs. BR3N401 delivered consistently higher recovery across all conditions while matching XP’s lot-to-lot consistency and inter-replicate stability.

High recovery efficiency

BR3N401 AcuGenix™ NGS DNA Clean Beads: Figure 3. Equivalent purification and size-selection performance to AMPure XP — **Figure 3. Equivalent purification and size-selection performance to AMPure XP.** At a 1.0× ratio across 5 replicates, BR3N401 showed a recovery rate approximately 6–7 percentage points higher than AMPure XP, with comparable reproducibility between batches.

Ratio-dependent Fragment Enrichment

BR3N401 AcuGenix™ NGS DNA Clean Beads: Figure 4. Tunable size selection with preferential long-fragment binding — **Figure 4. Tunable size selection with preferential long-fragment binding.**
**(A) Single-sided recovery assay.** A 2-kb DNA marker was purified with BR3N401 at incrementally increasing bead ratios (0.5×–2.2×). Electrophoresis revealed a stepwise shift in dominant fragment size, confirming ratio-dependent cutoff tuning and the beads' intrinsic bias toward larger DNA fragments.
**(B) Double-sided size-selection assay.** A 2-kb DNA marker was processed with BR3N401 using a two-step, double-sided selection workflow: a first bead ratio (1.0×–0.45×) was applied to bind and remove fragments above the upper cutoff, and a second ratio (0.1×–0.3×) was then added to the supernatant to bind and recover fragments within the desired size window. Electrophoresis showed a progressive upward shift of the recovered fragment range as the first ratio decreased, yielding discrete, narrowed band windows instead of the inclusive recovery observed in single-sided selection. Together, panels A and B demonstrate that BR3N401 supports both ratio-tunable single-sided recovery and precise windowed (double-sided) size selection, with consistent preferential binding toward longer DNA fragments.

Note: Lower bead ratios increase the minimum fragment size threshold for binding, the core principle of NGS library size selection.

Size-selection Parity with AMPure XP

Figure 5. Size-selection profile parity with AMPure XP. A 2-kb DNA marker was purified with BR3N401 or AMPure XP at identical bead ratios. Electrophoresis showed matching dominant band sizes and distribution patterns for both reagents across the full tested ratio range, confirming equivalent size-selection performance.

Note: Full-range parity confirms BR3N401 is fully compatible with all AMPure XP-dependent library preparation workflows.