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Dye-Based qPCR Master Mix · Cat. no. BR1E101

AcuGenix™ Robustart qPCR Master Mix (Universal ROX)

AcuGenix™ Robustart qPCR Master Mix (Universal ROX) is a dedicated reagent for Real-Time PCR using fluorescent dye.

Universal ROX
No ROX adjustment across instruments
Antibody hot start
Higher specificity
AcuGenix™ Robustart qPCR Master Mix (Universal ROX), BR1E101 product image

Product overview

AcuGenix™ Robustart qPCR Master Mix (Universal ROX) is a dedicated reagent for Real-Time PCR using fluorescent dye. The polymerase activity is completely blocked at room temperature by antibody modification, which can effectively inhibit non-specific amplification caused by primer non-specific annealing or primer dimer at room temperature and improve the specificity of amplification reaction. The optimized qPCR buffer greatly improves the amplification efficiency and detection sensitivity of qPCR reaction, and can obtain a good standard curve in a wide quantitative region for accurate quantification. This product contains universal ROX Reference Dye, compatible with most qPCR instruments, with no need to adjust the concentration of ROX on different instruments.

Features and applications

Features
  • High Specificity: Antibody-modified enzymes combined with optimized buffer deliver superior specificity.
  • Strong Fluorescent Signal: The fluorescent dye generates high-intensity signals for stable detection results.
  • Easy-to-Use: A universal ROX reference dye is adopted, compatible with various qPCR instruments. No ROX concentration adjustment is required across different instruments.
Applications
  • Differential gene expression analysis;
  • Absolute quantitative analysis.

Components

ComponentBR1E101-01
500 T (20 μL/T)
BR1E101-02
2500 T (20 μL/T)
2×AcuGenix™ Robustart qPCR Master Mix (Universal ROX)5×1 mL25×1 mL

Performance data

Comparison with Comparable Products

Supplier A   Supplier B   BR1E101

Gentier 48E

BR1E101 AcuGenix™ Robustart qPCR Master Mix (Universal ROX): Melt curve
Melt curve
BR1E101 AcuGenix™ Robustart qPCR Master Mix (Universal ROX): Amplification plot
Amplification plot

ABI 7500

BR1E101 AcuGenix™ Robustart qPCR Master Mix (Universal ROX): Melt curve
Melt curve
BR1E101 AcuGenix™ Robustart qPCR Master Mix (Universal ROX): Amplification plot
Amplification plot

Conclusion: The Biori BR1E101 reagent exhibits better compatibility across different platforms, with markedly superior amplification signals compared to Supplier A and Supplier B.

Shipping and storage

ConditionRecommendation
StorageStore at -20±5°C
ShippingRefer to product label and shipping documentation.

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