Cell Total Protein Extraction
Cell Total Protein Extraction Kit is designed for the extraction of total proteins from various animal cells, fungi, or bacterial samples.
Product overview
Cell Total Protein Extraction Kit is designed for the extraction of total proteins from various animal cells, fungi, or bacterial samples. The provided lysis buffer enables efficient extraction of total proteins, including those from cytoplasm, cell membranes, nuclei, nuclear matrix, mitochondria, endoplasmic reticulum, and Golgi apparatus. The resulting total protein samples can be utilized for protein immunoblotting, immunoprecipitation, co-immunoprecipitation (Co-IP), ELISA assays, protein analysis, and small-scale protein chromatographic purification, among other applications. The kit includes protein loading buffer and a protease inhibitor mixture. For phosphorylation sample preparation, the dedicated phosphatase inhibitor (Product Code: BR4C122) is recommended.
Features and applications
- The provided lysis buffer enables efficient extraction of total proteins, including those from cytoplasm, cell membranes, nuclei, nuclear matrix, mitochondria, endoplasmic reticulum, and Golgi apparatus.
- The kit includes protein loading buffer (5× SDS-PAGE Protein Loading Buffer) and a protease inhibitor mixture (Protease Inhibitor Cocktail, EDTA-Free (100×)).
- Two cell lysis methods: the vial lysis method (most effective) is suitable for all adherent cells; the centrifugation method is suitable for suspended cells and drug-treated cells.
- Simple protocol: add 1 mL of pre-cooled lysis buffer per 10 million cells and incubate on ice for 30 minutes.
- Extraction of total proteins from various animal cells, fungi, or bacterial samples.
- Protein immunoblotting, immunoprecipitation, co-immunoprecipitation (Co-IP), ELISA assays, protein analysis, and small-scale protein chromatographic purification.
- Phosphorylation sample preparation, together with the dedicated Phosphatase Inhibitor Cocktail (100×) (BR4C122).
Components
| Components | BR4C131-01 30 T |
|---|---|
| Lysis Buffer | 30 mL |
| 5× SDS-PAGE Protein Loading Buffer | 7.5 mL |
| Protease Inhibitor Cocktail, EDTA-Free (100×) | 0.3 mL |
Shipping and storage
| Condition | Recommendation |
|---|---|
| Storage | Store at -20±5°C. Mix thoroughly before use. The Lysis Buffer may be temporarily stored at 2-8°C after thawing. |
| Shipping | Ship frozen or under conditions that maintain the specified -20°C storage condition. |
| Handling | For research use only. Review the MSDS before use. |
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