Phosphatase Inhibitor Cocktail (100×)
Phosphatase Inhibitor Cocktail (100×) contains sodium fluoride, β-glycerophosphate, and sodium orthovanadate as phosphatase inhibitors for cell or tissue extracts.
Product overview
Samples such as cell or tissue extracts contain numerous endogenous proteases and phosphatases, which can readily lead to protein degradation or demodification in the extracts, thereby affecting subsequent protein detection. Therefore, adding appropriate protease and phosphatase inhibitors to extracts and similar samples is an effective method to prevent protein degradation and demodification. This product, Phosphatase Inhibitor Cocktail (100×), contains sodium fluoride, β-glycerophosphate, and sodium orthovanadate as phosphatase inhibitors. Among these, sodium fluoride serves as a reversible inhibitor for acid phosphatase; β-glycerophosphate acts as a reversible inhibitor for serine/threonine phosphatase; and sodium orthovanadate functions as a reversible inhibitor for alkaline phosphatase and tyrosine phosphatase.
Features and applications
- Contains three phosphatase inhibitors: sodium fluoride, β-glycerophosphate, and sodium orthovanadate.
- Sodium fluoride serves as a reversible inhibitor for acid phosphatase; β-glycerophosphate acts as a reversible inhibitor for serine/threonine phosphatase; and sodium orthovanadate functions as a reversible inhibitor for alkaline phosphatase and tyrosine phosphatase.
- 100× stock solution: add it to the lysis buffer at a ratio of 1:100, mix well, and it is ready for use.
- Preservation of protein phosphorylation during routine cell or tissue lysis and protein extraction.
- Phosphorylation sample preparation, for example together with Cell Total Protein Extraction (BR4C131).
- Add to lysis buffers such as RIPA Lysis Buffer (BR4C101–BR4C104) at a ratio of 1:100 before use.
Components
| Components | BR4C122-01 |
|---|---|
| Phosphatase Inhibitor Cocktail (100×) | 1 mL |
Shipping and storage
| Condition | Recommendation |
|---|---|
| Storage | Store at -20±5°C. |
| Shipping | Ship frozen or under conditions that maintain the specified -20°C storage condition. |
| Handling | For research use only. Review the MSDS before use. |
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