DAB Peroxidase Substrate Kit
DAB Peroxidase Substrate Kit is a colorimetric substrate kit developed for horseradish peroxidase (HRP) detection.
Product overview
DAB Peroxidase Substrate Kit is a colorimetric substrate kit developed for horseradish peroxidase (HRP) detection. The kit contains all reagents required for HRP detection and is suitable for staining PVDF and NC membranes used in Western blot assays. DAB (3,3′-diaminobenzidine tetrahydrochloride) is a commonly used substrate for HRP. In the presence of HRP, DAB is oxidized to form a brown precipitate that is insoluble in water and ethanol. HRP immobilized at the target protein site after immunoreaction catalyzes the conversion of DAB into a brown reaction product, allowing the location and expression level of the target protein to be assessed based on the color reaction.
Features and applications
- Simplified components.
- Easy-to-operate.
- Low background.
- The kit contains all reagents required for HRP detection and is suitable for staining PVDF and NC membranes used in Western blot assays.
Components
| Components | Volume |
|---|---|
| 20×DAB-A | 3 mL |
| 1×DAB-B | 60 mL |
Performance data
Color-development test
Conclusion: The detection sensitivity of Biori DAB Kit is slightly higher than that of Supplier N, with clear color development and clean background.
Shipping and storage
| Condition | Recommendation |
|---|---|
| Storage | Store at 2-8°C,protected from light. |
| Shipping | Shipped with ice packs |
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