10× Fast Transfer Buffer

10× Fast Transfer Buffer is a high-efficiency, safe, and non-toxic transfer buffer developed for rapid wet transfer in Western blot workflows.

20–40 min

Wet transfer, internal ice block only

Non-toxic

No hazardous components

Tris-Gly, HEPES, Bis-Tris

Gel system compatibility

Product overview

10× Fast Transfer Buffer is a high-efficiency, safe, and non-toxic transfer buffer developed for rapid wet transfer in Western blot workflows. This product is supplied as a 10× concentrate and should be diluted with deionized water and ethanol to a 1× working solution before use. The formulation does not contain highly toxic components, and ethanol can be used in place of methanol during buffer preparation, making the workflow safer and more environmentally friendly. This buffer enables convenient operation and can complete membrane transfer in 20-40 min with low heat generation. In most cases, an internal ice pack in the transfer unit is sufficient and an external ice bath is not required. It is also suitable for proteins spanning a wide molecular-weight range, helping support simultaneous transfer of both small and large proteins on the same membrane. This product is compatible with Tris-Glycine, HEPES, and Bis-Tris gel systems.

Features and applications

Features

Eco-Friendly & Safe: Contains no toxic components.
Rapid & Convenient: Completes wet transfers in just 20–40 minutes. Requires only an internal ice block during the transfer process, eliminating the need for an external ice bath.
Broad Compatibility: Highly effective for proteins across a wide range of molecular weights. Fully compatible with various gel systems, including Tris-Glycine, HEPES, and Bis-Tris.

Applications

Protein transfer from polyacrylamide gels to nitrocellulose (NC) or PVDF membranes via wet electroblotting.
Routine Western blot workflows requiring rapid and highly efficient transfer.