ECL Chemiluminescent Substrate Kit

ECL Chemiluminescent Substrate Kit is a chemiluminescent detection kit that contains all reagents required for the detection of horseradish peroxidase (HRP).

pg-level

Antigen detection

4 weeks at 37 °C

Signal intensity maintained

Stable signal

Comparable to Supplier N

Product overview

ECL Chemiluminescent Substrate Kit is a chemiluminescent detection kit that contains all reagents required for the detection of horseradish peroxidase (HRP) and is suitable for chemiluminescent detection in Western blot applications, enabling rapid detection of pg-level antigens. This product can be used to detect antibodies directly or indirectly labeled with HRP and their associated antigens. The principle is as follows: after electrophoresis, proteins or nucleic acids are transferred onto a blotting membrane. The target protein on the membrane is recognized by a primary antibody and an HRP-labeled secondary antibody, or nucleic acids are bound directly or indirectly by an HRP-labeled probe. HRP catalyzes the decomposition of hydrogen peroxide and the oxidation of luminol, generating an excited-state intermediate that emits photons during decay. This product can be used for the detection of proteins and other biomacromolecules immobilized on membranes and can be detected by X-ray film exposure or with a chemiluminescence imaging system.

Features and applications

Features

Rapid detection of pg-level antigens.
Stable luminescent signals.
High cost-effectiveness.

Applications

Western blot chemiluminescence signal development.
For the detection of antibodies directly or indirectly conjugated with horseradish peroxidase (HRP) and their associated antigens.

Components

Component	BR4D602-01 100 mL	BR4D602-02 500 mL
Solution A	50 mL	250 mL
Solution B	50 mL	250 mL

Performance data

WB detection of housekeeping protein α-tubulin

Anti α-tubulin Mouse Monoclonal Antibody, dilution ratio 1:5000
HRP-conjugated goat anti-mouse IgG, dilution ratio 1:5000

Conclusion: The same antigen sample was loaded with serially diluted concentrations for WB analysis. The signal intensity detected by Biori ECL Kit is comparable to that of Supplier N.

WB detection of housekeeping protein GAPDH

Anti GAPDH Mouse Monoclonal Antibody, dilution ratio 1:5000
HRP-conjugated goat anti-mouse IgG, dilution ratio 1:5000

Conclusion: The same antigen sample was loaded with serially diluted concentrations for WB analysis. The signal intensity detected by Biori ECL Kit is comparable to that of Supplier N.

WB detection of protein with His tag

Anti His-Tag Mouse Monoclonal Antibody, dilution ratio 1:5000
HRP-conjugated goat anti-mouse IgG, dilution ratio 1:5000

Conclusion: The same antigen sample was loaded with serially diluted concentrations for WB analysis. The signal intensity detected by Biori ECL Kit is comparable to that of Supplier N.

Rapid loading detection of HRP-containing samples

Conclusion: When detecting the same sample with serial dilutions, Biori ECL Kit exhibits comparable signal intensity to Supplier N.

Stability evaluation

Conclusion: Following 4-week accelerated test at 37 °C, Biori ECL Kit maintains consistent detection signal intensity compared with low-temperature stored samples, showing outstanding stability.