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Product Catalog
mRNA Capping Kit, BP-04, is designed for enzymatic addition of 5′ cap structures to RNA after in vitro transcription. The workflow uses capping enzymes and methyl-donor/cofactor components described in the manual to support preparation of capped mRNA for research and process-development applications.
Features
- Capping-related reagent for mRNA production and post-transcriptional processing workflows.
- Supplied format and storage conditions are described in the instruction manual.
- Supports enzymatic capping or methyl donor use where stated by the source document.
Application
- mRNA capping workflows.
- Research and manufacturing process development for capped RNA products.
Components
| Components | Cat. No. | Volume |
|---|---|---|
| Vaccinia Capping Enzyme | BP-E05 | 50uL |
| 2′-O-Methyltransferase | BP-E06 | 50uL |
| 10× Capping Buffer | BP-AS-12 | 100uL |
| GTP Solution (10mM) | BP-AS-02 | 50uL |
| SAM (32mM) | BP-AS-06 | 25uL |
| RNase Inhibitor (40 U/uL) | BP-E02 | 50uL |
| RNase-free ddH2O | BP-AS-11 | 1mL |
Shipping and Storage
| Condition | Recommendation |
|---|---|
| Storage | Store at -20±5°C. |
| Shipping | Ship frozen on dry ice or with validated frozen cold-chain packaging that maintains the specified frozen storage condition. |
| Handling | Avoid repeated freeze-thaw cycles; mix thoroughly after thawing where applicable. |
Data
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