mRNA Capping Kit, BP-04
Enzymatic kit for post-transcriptional mRNA capping.
Product overview
The mRNA Capping Kit independently developed by Biori Biotech contains Vaccinia Capping Enzyme (VCE) and mRNA Cap 2'-O-Methyltransferase (2OM). Vaccinia Capping Enzyme efficiently adds an m7G cap to the 5' end of mRNA, thereby producing RNA with a Cap0 structure. 2'-O-Methyltransferase adds a methyl group to the 2'-O position of the first nucleotide following the cap structure of Cap0 RNA, forming RNA with a Cap1 structure. This kit enables simultaneous capping by VCE and 2OM in a single-tube reaction, directly yielding Cap1 RNA. It can significantly enhance the intracellular stability of mRNA and promote translation efficiency.
Features and applications
- Contains Vaccinia Capping Enzyme (VCE) and mRNA Cap 2'-O-Methyltransferase (2OM).
- Simultaneous capping by VCE and 2OM in a single-tube reaction, directly yielding Cap1 RNA.
- A 20 μL capping system is recommended per 50 pmol of RNA; incubation at 37°C for 1 hour is sufficient to complete capping.
- Post-transcriptional enzymatic capping of in vitro transcribed RNA to produce Cap1 mRNA.
- Research and manufacturing process development for capped RNA products.
Components
| Components | Cat. No. | Volume |
|---|---|---|
| Vaccinia Capping Enzyme | BP-E05 | 50µL |
| 2'-O-Methyltransferase | BP-E06 | 50µL |
| 10× Capping Buffer | BP-AS-12 | 100µL |
| GTP Solution (10mM) | BP-AS-02 | 50µL |
| SAM (32mM) | BP-AS-06 | 25µL |
| RNase Inhibitor (40 U/µL) | BP-E02 | 50µL |
| RNase-free ddH2O | BP-AS-11 | 1mL |
Performance data
1 Performance test
Description: Using LC-MS, the cap rate of the samples treated with the kit was measured to evaluate the performance of the kit.
Conclusion: Capping efficiency of ≥95% can be achieved using this kit.
2 Carry out cell expression verification on the samples processed with the kit
Description: The cell expression of GFP treated with the reagent kit was verified, and the capping activity of the reagent kit was reflected by the fluorescence signal.
Conclusion: The fluorescence intensity of mRNA-GFP after treatment with the kit was high, indicating that the kit has a strong capping activity.
Shipping and storage
| Condition | Recommendation |
|---|---|
| Storage | Store at -20±5°C. |
| Shipping | Ship frozen on dry ice or with validated frozen cold-chain packaging that maintains the specified frozen storage condition. |
| Handling | Avoid repeated freeze-thaw cycles; mix thoroughly after thawing where applicable. |
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