Neoscript Hot Start Reverse Transcriptase-V2
Neoscript Hot Start Reverse Transcriptase-V2, E43, is supplied for cDNA synthesis, detection of various RNA pathogens.
Product overview
Neoscript Hot Start Reverse Transcriptase-V2 represents a high-activity reverse transcriptase acquired via the targeted modification of Neoscript RTase utilizing a rational screening platform. It features superior enzymatic activity, thermal tolerance and enhanced inhibitor resistance, making it applicable to high-temperature and rapid reverse transcription.
Available versions
| Cat. no. | Product name | Formulation | Residual DNA level |
|---|---|---|---|
| E43 | Neoscript Hot Start Reverse Transcriptase-V2 | Liquid (with glycerol) | Standard |
| FE43 | Neoscript Hot Start Reverse Transcriptase (Glycerol-Free)-V2 | Glycerol-free | Standard |
| E43-NA | Neoscript Hot Start Reverse Transcriptase-Low DNA-V2 | Liquid (with glycerol) | Low Residual DNA |
| FE43-NA | Neoscript Hot Start Reverse Transcriptase-Low DNA (Glycerol-Free)-V2 | Glycerol-free | Low Residual DNA |
Features and applications
- Hot-start modification suppresses reverse-transcription activity at low temperature; full activity is released at 50°C.
- The reverse transcription temperature range is 50-55°C.
- Higher stability.
- Strong tolerance to inhibitors from samples such as blood and swabs.
- cDNA synthesis, detection of various RNA pathogens.
- Gene expression analysis.
- Rapid amplification, direct amplification, nucleic acid detection POCT.
Components
| Component | Specification/Notes |
|---|---|
| Neoscript Hot Start Reverse Transcriptase-V2 | 200U/μL |
| 5×First-Strand Buffer (optional) | 5× |
Performance data
1. Rapid PCR Program Test
Amplification Curves
| Standard PCR Program | ||||
| Channel | FAM | VIC | ROX | CY5 |
|---|---|---|---|---|
| Amplification Curves |  |  |  |  |
| Rapid PCR Program | ||||
| Channel | FAM | VIC | ROX | CY5 |
| Amplification Curves |  |  |  |  |
| Rapid PCR Program- Standard PCR Protocol (ΔCt) | ||||
| Dilution Concentration (copies/T) | FAM | VIC | ROX | CY5 |
|---|---|---|---|---|
| 50 | 0.89 | 0.96 | 0.70 | 1.03 |
| 500 | 0.47 | 0.99 | 0.55 | 0.97 |
Conclusion 1: The E43 is compatible with the rapid PCR program. Compared with the conventional amplification protocol (1 h 19 min), the rapid PCR program (30 min 16 s) yielded ΔCt values ≤ 1.03 across all channels.
2. IC50 Test
Conclusion 1: In the IC50 assay, E43 outperformed E13 and the Supplier A reference product.
3. Determination of Reverse-Transcription Activity
Conclusion 1: E43 exhibited low reverse-transcription activity at 30 °C; at 50 °C, its activity increased significantly, indicating full release of enzyme activity.
Shipping and storage
| Condition | Recommendation |
|---|---|
| Shipping Conditions | -20±5°C |
| Storage Conditions | -20±5°C |
| Shelf Life | 24M |
Downloads
Neoscript Hot Start Reverse Transcriptase (Glycerol-Free)-V2 (FE43)
Instruction Manual (PDF)COA (PDF)MSDS (PDF)Neoscript Hot Start Reverse Transcriptase-Low DNA-V2 (E43-NA)
Instruction Manual (PDF)COA (PDF)MSDS (PDF)Neoscript Hot Start Reverse Transcriptase-Low DNA (Glycerol-Free)-V2 (FE43-NA)
Instruction Manual (PDF)COA (PDF)MSDS (PDF)
Need this product lyophilized, in bulk, or in a custom format?
The feasibility assessment is free of charge. Send the assay brief and target format.
