Pre-treatment Kit for mRNA Poly A Distribution Detection, BP-11
Prepares mRNA samples for poly(A) distribution analysis.
Product overview
Pre-treatment Kit for mRNA Poly A Distribution Detection, BP-11, prepares mRNA samples for downstream poly(A) tail distribution analysis. The kit supports analytical workflows described in the manual, including LC-MS-oriented sample preparation for poly(A) length and heterogeneity assessment.
Features and applications
- mRNA quality-control or sample pre-treatment tool for process analytical workflows.
- Supplied components and storage conditions are listed in the instruction manual.
- Supports detection, ladder sizing, or sample preparation workflows as described by the source document.
- For sample preparation prior to the detection of poly(A) distribution in capped mRNA.
Components
| Components | Volume | Storage | |
|---|---|---|---|
| Box 1 | T Reagent | 60 μL | -20±5°C |
| 10×T Buffer | 120 μL | ||
| Box 2 | Magnetic Beads | 1 mL | 2-8°C |
| Buffer I | 6 mL | ||
| Buffer II | 20 mL | ||
| EB Buffer | 1 mL×2 |
Performance data
1 Functional test of the kit
Description: The treated samples after enzymatic digestion and the recovered samples were verified using denaturing electrophoresis.
Conclusion: After digestion with RNase T1 using the kit, there were still considerable impurities. However, following purification and recovery, the purity of the Poly(A) tail band was significantly improved.
2 LC-MS analysis for determining the distribution of Poly(A)
Description: The recovered samples were subjected to LC-MS analysis to determine the distribution of Poly(A).
Conclusion: The ion current within the target peak exhibited a trend toward normal distribution. Through deconvolution and spectral analysis, a standard normal or near-normal distribution of the Poly(A) tail lengths could be obtained.
Table 1 Recommended reaction system.
| Components | Volume |
|---|---|
| T Reagent | 5 μL |
| RNA | 50 μg |
| 10×T Buffer | 5 μL |
| RNase-free ddH2O | to 50 μL |
Shipping and storage
| Condition | Recommendation |
|---|---|
| Storage | Box 1 is stored at -20±5°C and Box 2 at 2-8°C. Do not freeze-thaw the magnetic beads. |
| Shipping | Ship with split cold-chain packaging: frozen components at -20°C on dry ice and refrigerated components at 2-8°C with cold packs. |
| Handling | Avoid repeated freeze-thaw cycles; mix thoroughly after thawing where applicable. |
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