RNase Residual Quantitative Detection Kit, BP-06
Quantifies residual RNase activity in mRNA workflows.
Product overview
RNase Residual Quantitative Detection Kit, BP-06, is designed for quantitative detection of residual RNase in mRNA and IVT-related workflows. The kit supports quality-control testing where nuclease contamination may affect RNA integrity.
Features and applications
- (1) Based on enzymatic activity, designed for residual RNase detection.
- (2) High sensitivity: LOD down to 0.078 pg RNase.
- (3) Rigorous QC ensures sensitivity, inter/intra assay variation, accuracy.
- (1) Biopharmaceutical and biological product QC: process residual detection and raw material/consumable monitoring;
- (2) Molecular biology experiment support;
- (3) Production and lab environment monitoring.
Components
| Product Name | Cat. No. | Components | Volume |
|---|---|---|---|
| RNase Residual Quantitative Detection Kit | BP-AS-18 | RNase A(10 mg/mL) | 10 μL |
| BP-AS-19 | RNase Substrate | 1 mL | |
| BP-AS-20 | 10× Reaction Buffer | 1 mL | |
| BP-AS-21 | RNase-free ddH2O | 1 mL×5 |
Performance data
1 Assay principle
Description: Schematic diagram of the Biori RNase Residual Quantitative Detection Kit.
2 Sensitivity
Description: The sensitivity of the kit was evaluated by detecting varying amounts of RNase A.
Conclusion: The kit exhibits a sensitivity of 0.078 pg RNase.
3 Fluorescence intensity plot
Description: Time course of fluorescence intensity for the detection of various amounts of RNase A using this kit over a 60 minutes period.
Conclusion: The figure shows the changes in fluorescence intensity of RNase A over a 60-minute period.
4 Standard curve
Description: Establishing the standard curve by gradient dilution of RNase A standard.
Conclusion: The RNase A standard were subjected to gradient dilution for absorbance measurement. The data were processed using a Linear fitting. The results showed that the linear R2 value was greater than 0.99.
Shipping and storage
| Condition | Recommendation |
|---|---|
| Storage | Store at -20±5°C. Protect RNase Substrate (BP-AS-19) from light. |
| Shipping | Ship frozen on dry ice or with validated frozen cold-chain packaging that maintains the specified frozen storage condition. |
| Handling | Protect from light and avoid unnecessary freeze-thaw cycles where applicable. |
Downloads
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