Robustart Fast Taq DNA Polymerase-V2 is a hot-start fast amplification DNA polymerase developed by Biori. The enzyme effectively suppresses non-specific amplification caused by primer misannealing or primer-dimer formation during PCR setup and amplification.
This enzyme features rapid DNA amplification capability and improved tolerance to common PCR inhibitors, enabling reliable amplification even from low-concentration templates. Robustart Fast Taq DNA Polymerase-V2 is suitable for conventional PCR, rapid PCR assays, multiplex PCR, and nucleic acid detection applications.
Robustart Fast Taq DNA Polymerase-V2, E20
Features
- Antibody-modified hot-start activation at 95°C for 1–5 minutes.
- Rapid DNA amplification speed up to 1 kb per 10 seconds.
- High specificity with strong suppression of non-specific amplification.
- Strong tolerance to PCR inhibitors from blood, swabs, and other sample sources.
- Suitable for rapid PCR and multiplex amplification applications.
Application
- Conventional PCR amplification
- Quantitative PCR (qPCR) assays
- Rapid PCR amplification
- Direct PCR amplification
- Point-of-care nucleic acid testing (POCT)
Components
| No. | Component | Notes |
|---|---|---|
| 1 | 5 U/µL Robustart Fast Taq DNA Polymerase-V2 | — |
| 2 | 10× PCR Buffer II (Mg²⁺-free) | Optional |
| 3 | 25 mM MgCl₂ | Optional |
Note: The 10× PCR Buffer II (Mg²⁺-free) does not contain dNTPs or Mg²⁺. Please add dNTPs and MgCl₂ when preparing the PCR reaction system.
Shipping and Storage
| Condition | Recommendation |
|---|---|
| Shipping | Ship on dry ice. |
| Storage | Store at −20 ± 5°C. |
| Handling | Mix thoroughly before use and avoid repeated freeze–thaw cycles. |
Data
Experimental validation data will be updated soon.
