Robustart Fast Taq DNA Polymerase-V2

Fast hot-start Taq DNA polymerase optimized for rapid PCR amplification and inhibitor-tolerant nucleic acid detection.

Lyo-ready FE20 Low-residual DNA E20-NA Lyo-ready + Low-residual DNA FE20-NA

≥ 1 kb / 10 s

Amplification rate

Hot start

Antibody-modified, inactive at 40 °C

20 freeze-thaw cycles

Plus 14 days at 37 °C, no performance loss

Product overview

Robustart Fast Taq DNA Polymerase-V2 is a hot-start fast amplification DNA polymerase developed by Biori. The enzyme effectively suppresses non-specific amplification caused by primer misannealing or primer-dimer formation during PCR setup and amplification.

This enzyme features rapid DNA amplification capability and improved tolerance to common PCR inhibitors, enabling reliable amplification even from low-concentration templates. Robustart Fast Taq DNA Polymerase-V2 is suitable for conventional PCR, rapid PCR assays, multiplex PCR, and nucleic acid detection applications.

Available versions

Cat. no.	Product name	Formulation	Residual DNA level
E20	Robustart Fast Taq DNA Polymerase-V2	Liquid (with glycerol)	Standard
FE20	Robustart Fast Taq DNA Polymerase-V2 (Glycerol-Free)	Glycerol-free	Standard
E20-NA	Robustart Fast Taq DNA Polymerase-V2-Low DNA	Liquid (with glycerol)	Low Residual DNA
FE20-NA	Robustart Fast Taq DNA Polymerase-V2-Low DNA (Glycerol-Free)	Glycerol-free	Low Residual DNA

Features and applications

Features

Antibody modification, 95°C, 30s-1min hot start.
The amplification speed is fast, no less than 1kb per 10 seconds.
Highly resistant to inhibitors from sources such as blood and swabs.

Applications

Endpoint method, Quantitative PCR.
Rapid amplification, direct amplification, nucleic acid Application of POCT (Point-of-Care Testing).

Components

Component	Specification/Notes
Robustart Fast Taq DNA Polymerase-V2	5 U/μL
10×PCR Buffer II (Mg2+ free) (optional)	Does not contain dNTPs or Mg²⁺.
MgCl2 (optional)	25mM

Performance data

1. Accelerated Stability Test

Channel	VIC	ROX	CY5
-20°C control 37°C 7 d 37°C 14 d
Amplification Curves

Conclusion 1: After the E20 enzyme was subjected to accelerated aging at 37°C for 7 and 14 days, its amplification performance was consistent with that of the unaccelerated reagent, indicating that it remained stable.

2. Freeze-Thaw Stability Test

Channel	VIC	ROX	CY5
Freeze-Thaw: 0 cycles 5 cycles 10 cycles 15 cycles 20 cycles
Amplification Curves

Conclusion 1: After 5, 10, 15, and 20 freeze-thaw cycles, E20 showed amplification performance consistent with that of the reagent without freeze-thaw treatment and remained stable.