Tth DNA Polymerase · Cat. no. E17
Robustart Tth DNA Polymerase
Robustart Tth DNA Polymerase, E17, is supplied for quantitative PCR.
60–70 °C RT
With Mn2+ for complex RNA templates
Pol + RT
Both activities in one enzyme
Inhibitor tolerant
Designed for crude samples
Product overview
Robustart Tth DNA Polymerase is a hot-start Tth enzyme that has 5'-3' DNA template-dependent polymerase activity, making it suitable for various PCR reactions. It is intended for quantitative PCR.
Available versions
Features and applications
Features
- Antibody-modified hot-start enzyme with a step above 90°C for 30 seconds to 1 minute.
- DNA polymerase and reverse-transcriptase activities.
- In the presence of Mn2+, reverse transcription can be performed at 60–70°C for complex RNA templates, helping reduce nonspecific reactions.
- Designed to tolerate inhibitors.
Applications
- Quantitative PCR.
- Multiplex PCR, genotyping, and high-sensitivity virus detection.
Components
| Component | Specification / Notes |
|---|---|
| 5 U/µL Robustart Tth DNA Polymerase | 5U/μL |
| 10×Tth RT Buffer Basic (Mn2+ free) (optional) | Included component; refer to the instruction manual |
| 25 mM Mn (OAc)2 (optional) | Included component; refer to the instruction manual |
Shipping and storage
| Condition | Recommendation |
|---|---|
| Shipping Temperature | Ice pack transportation (2-8°C) |
| Storage Temperature | -20±5°C |
| Shelf Life | 24M |
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