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Robustart Tth DNA Polymerase is a hot-start Tth enzyme that has 5'-3' DNA template-dependent polymerase activity, making it suitable for various PCR reactions. It is intended for quantitative PCR.
Features
- Antibody modification,above 90°C,hot
- start for 30s-1min.
- Bifunctional activities of polymerase and reverse transcriptase.
- In the presence of Mn2+,reverse
- transcription can be performed at a high
- temperature of 60-70°C,which has higher
- reverse transcription efficiency for complex RNA templates and reduces non-specificity.
- Strong tolerance to inhibitors.
Application
- Quantitative PCR.
- Multiplex PCR,genotyping detection,high-sensitivity virus detection.
- Suitable for storage at 2-8°C.
Components
| Component | Specification / Notes |
|---|---|
| 5 U/µL Robustart Tth DNA Polymerase | 5U/μL |
| 10×Tth RT Buffer Basic (Mn2+ free) (optional) | Included component; refer to the instruction manual |
| 25 mM Mn (OAc)2 (optional) | Included component; refer to the instruction manual |
Shipping and Storage
| Condition | Recommendation |
|---|---|
| Shipping Temperature | Ice pack transportation(2-8℃) |
| Storage Temperature | -20±5℃ |
| Shelf Life | 24M |
Data
Coming soon.