T7 RNA Polymerase GMP-grade

Recombinant T7 RNA polymerase for IVT RNA synthesis.

9 days at 37 °C

Transcription yield maintained

High integrity

Across template lengths by CE

Product overview

T7 RNA Polymerase GMP-grade is an in-house developed and multi-step purified recombinant T7 RNA polymerase manufactured by Biori Biotech to GMP standards. It exhibits high transcriptional catalytic activity across diverse template types and nucleotide substrates. Under appropriate reaction buffer conditions, this enzyme enables efficient synthesis of large quantities of RNA from DNA templates. T7 RNA Polymerase GMP-grade specifically recognizes the T7 promoter sequence (5'-TAATACGACTCACTATAG-3') and initiates transcription from the G residue at this site, converting downstream DNA sequences into single-stranded RNA. Using natural or modified nucleotides as substrates, a single 20 μL in vitro transcription reaction can yield more than 200 μg of RNA.

Available versions

Cat. no.	Product name	Formulation
GMP-BP-E01-5K	T7 RNA Polymerase GMP-grade
GMP-BP-E01-50K	T7 RNA Polymerase GMP-grade
GMP-BP-E01-500K	T7 RNA Polymerase GMP-grade

Features and applications

Features

Recombinant T7 RNA polymerase expressed in E. coli and manufactured to GMP standards; activity: 50 U/μL.
Unit definition: one unit (U) is defined as the amount of enzyme required to incorporate 1 nmol of NTP into RNA in 1 hour at 37°C, pH 8.0.
Quality control: protein purity ≥95%; free of exogenous DNase, RNase, exonuclease, and endonuclease activity; residual host-cell DNA ≤100 pg/mg; residual host-cell protein <50 ppm; bacterial endotoxin <5 EU/mL.

Applications

In vitro transcription and mRNA process development workflows.
RNA synthesis using natural or modified nucleotides as substrates.

Components

Components	Cat. No.	Quantity	Volume
T7 RNA Polymerase GMP-grade (50 U/μL)	GMP-BP-E01-5K	5 KU	100 μL
	GMP-BP-E01-50K	50 KU	1 mL
	GMP-BP-E01-500K	500 KU	10 mL

Performance data

1 Template compatibility test

Description: Verify the compatibility of T7 RNA polymerase with templates of varying lengths during transcription.

GMP-BP-E01 T7 RNA Polymerase GMP-grade: Figure 1 Result for transcriptional products of varying lengths — **Figure 1** Result for transcriptional products of varying lengths

Conclusion: During transcription of linearized templates of different lengths, transcripts produced using T7 RNA Polymerase GMP-grade (Cat. No. GMP-BP-E01) consistently maintained good integrity.

2 Transcription product integrity test

Description: Capillary electrophoresis for assessing the integrity of transcriptional products.

GMP-BP-E01 T7 RNA Polymerase GMP-grade: Figure 2 Transcriptional product integrity result — **Figure 2** Transcriptional product integrity result

Conclusion: CE data showed that transcripts produced using T7 RNA Polymerase GMP-grade had high integrity.

3 Product stability test

Description: Assessment of the stability of T7 RNA Polymerase GMP-grade under different temperatures based on transcriptional yield.

GMP-BP-E01 T7 RNA Polymerase GMP-grade: Figure 3 Transcriptional product yields at different temperatures — **Figure 3** Transcriptional product yields at different temperatures

Conclusion: After T7 RNA Polymerase GMP-grade was held at 37°C, 4°C, and -20°C for 9 days, transcription performance still maintained a high yield.

Shipping and storage

Condition	Recommendation
Storage	Store at -20±5°C.
Shipping	Ship frozen on dry ice or with validated frozen cold-chain packaging that maintains the specified frozen storage condition.
Handling	Avoid repeated freeze-thaw cycles; mix thoroughly after thawing where applicable.