AcuGenix™ Lyophilization Protectant-9

AcuGenix™ Lyophilization Protectant-9, AS412-9, is supplied for lyophilization protectant and excipient.

4× concentrate

6.25 µL per 25 µL reaction, 1× final

1 year at RT

Lyophilized FM5144 / FM2181-4 stability data

55 °C · 30 days

Accelerated stability after freeze-drying

Product overview

During the lyophilization process, the sample is exposed to cryogenic freezing, vacuum and dehydration. Lyophilization Protective Agent is a kind of substance that can increase the structural stability of lyophilized products, protect the active components in the process of lyophilization, prolong sample storage and provide good solubility for lyophilized samples.

Features and applications

Features

It helps to enhance the structural stability of the freeze-dried product, and during the freeze drying process, it can protect the active components of the sample, thereby extending the storage period of the sample.
It can provide excellent solubility for freeze dried samples, and the samples can be re-solubilized quickly.

Applications

Lyophilization protectant and excipient.
Used for PCR amplification reagents and raw material freeze-drying.

Components

Component	Specification/Notes
AcuGenix™ Lyophilization Protectant-9	4×

Performance data

1. Freeze-Thaw Stability Test

Channel	FAM	VIC	ROX	CY5
— FM2181-4: 0 Freeze-Thaw Cycles — FM2181-4: 5 Freeze-Thaw Cycles — FM2181-4: 10 Freeze-Thaw Cycles — FM2181-4: 15 Freeze-Thaw Cycles — FM2181-4: 20 Freeze-Thaw Cycles
Amplification Curves

Conclusion 1: After 5, 10, 15, and 20 freeze-thaw cycles, the amplification performance of the FM2181-4 premix reagent containing AcuGenix™ Lyophilization Protectant-9 (AS412-9) was consistent with that of the reagent not subjected to freeze-thaw cycling, demonstrating stability.

2. High-Temperature Accelerated Stability Test

Channel	FAM	VIC	ROX	CY5
Cat.: FM5144
Liquid Control Lyophilized Sample, accelerated at 55°C for 30 days
Amplification Curves	/
Cat.: FM2181-4
Liquid Control Lyophilized Sample, accelerated at 55°C for 30 days
Channel	FAM	VIC	ROX	CY5
Amplification Curves

Conclusion 1: After lyophilization with AS412-9, the FM5144 system remained stable after accelerated storage at 55°C for 30 days; amplification performance was consistent with the liquid control.

Conclusion 2: After lyophilization with AS412-9, the FM2181-4 system remained stable after accelerated storage at 55°C for 30 days; amplification performance was consistent with the liquid control.

3. Room-Temperature Accelerated Stability Test

Channel	FAM	VIC	ROX	CY5
Cat.: FM5144
Liquid Control Lyophilized Sample at room-temperature for one year
Amplification Curves	/
Cat.: FM2181-4
Liquid Control Lyophilized Sample at room-temperature for 18 months
Channel	FAM	VIC	ROX	CY5
Amplification Curves

Conclusion 1: After lyophilization with AS412-9, the FM5144 system remained stable after accelerated storage at room-temperature for one year; amplification performance was consistent with the liquid control.

Conclusion 2: After lyophilization with AS412-9, the FM2181-4 system remained stable after accelerated storage at room-temperature for 18 months; amplification performance was consistent with the liquid control.