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Product Catalog
CircRNA Synthesis Kit, BP-13, is designed for circular RNA synthesis workflows using the components and protocol described in the instruction manual. It supports preparation of covalently closed circular RNA from suitable RNA substrates for research and process-development applications.
Features
- Kit format for RNA or mRNA synthesis workflow steps.
- Supplied with workflow-specific enzymes, buffers, nucleotides, or related reagents listed in the instruction manual.
- Designed for in vitro transcription, co-transcriptional synthesis, or circular RNA synthesis workflows as applicable.
Application
- RNA and mRNA synthesis workflow development.
- Preparation of RNA products using the protocol and components described in the instruction manual.
Components
| Components | Cat. No. | Volume |
|---|---|---|
| ATP Solution (100mM) | BP-AS-01 | 100uL |
| GTP Solution (100mM) | BP-AS-02 | 100uL |
| CTP Solution (100mM) | BP-AS-03 | 100uL |
| UTP Solution (100mM) | BP-AS-04 | 100uL |
| T7RNA Polymerase | BP-E01 | 100uL |
| RNase Inhibitor (40 U/uL) | BP-E02 | 100uL |
| Inorganic Pyrophosphatase (0.1 U/uL) | BP-E03 | 100uL |
| 10× Transcription Buffer | BP-AS-08 | 100uL |
| Precipitation Reagent | BP-AS-10 | 1mL |
| DNase I (2 U/uL) | BP-E04 | 50uL |
| 10× DNase IReaction Buffer | BP-AS-13 | 100uL |
| RNase-freedd HO 2 | BP-AS-11 | 1mL |
| 10× Circular Buffer | BP-AS-30 | 1mL |
| RNase R (20 U/uL) | BP-E08 | 50uL |
| 10× RNase RBuffer | BP-AS-14 | 1mL |
| RNase RDilution Buffer | BP-AS-15 | 1mL |
Shipping and Storage
| Condition | Recommendation |
|---|---|
| Storage | Store at -20±5°C. |
| Shipping | Ship frozen on dry ice or with validated frozen cold-chain packaging that maintains the specified frozen storage condition. |
| Handling | Avoid repeated freeze-thaw cycles; mix thoroughly after thawing where applicable. |
Data
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