T7 High-Efficiency Transcription Kit, BP-01
High-efficiency IVT kit for RNA synthesis from T7 templates.
Product overview
Developed independently by BIORI, the T7 High-Efficiency Transcription Kit is optimized for transcription reaction systems across different templates and nucleotide types. This kit enables efficient production of high yields of RNA from DNA templates in vitro. Using T7 promoter-specific RNA polymerase, the kit transcribes high-yield single-stranded RNA from plasmids or PCR products containing the T7 promoter sequence, using natural or modified nucleotides as substrates. By including Cap0, Cap1, or other cap analogs in the reaction, capped full-length mRNA can be synthesized directly via co-transcription.
Features and applications
- Transcribes high-yield single-stranded RNA from plasmids or PCR products containing the T7 promoter sequence, using natural or modified nucleotides as substrates.
- Capped full-length mRNA can be synthesized directly via co-transcription by including Cap0, Cap1, or other cap analogs in the reaction.
- A 20 μL in vitro transcription system is recommended; through process design and optimization, the kit supports gram-scale RNA production.
- RNA structural and functional analysis.
- Preparation of RNA for circularization or for enzymatic capping with Vaccinia Capping Enzyme GMP-grade (GMP-BP-E05) and mRNA Cap 2'-O-Methyltransferase GMP-grade (GMP-BP-E06).
- Co-transcriptional synthesis of capped mRNA for functional studies.
Components
| Components | Cat. No. | Volume |
|---|---|---|
| ATP Solution (100 mM) | BP-AS-01 | 100 μL |
| GTP Solution (100 mM) | BP-AS-02 | 100 μL |
| CTP Solution (100 mM) | BP-AS-03 | 100 μL |
| UTP Solution (100 mM) | BP-AS-04 | 100 μL |
| T7 RNA Polymerase | BP-E01 | 100 μL |
| RNase Inhibitor (40 U/μL) | BP-E02 | 100 μL |
| Inorganic Pyrophosphatase (0.1 U/μL) | BP-E03 | 100 μL |
| 10× Transcription Buffer | BP-AS-08 | 100 μL |
| Control Template+ | BP-AS-09 | 5 μL |
| Precipitation Reagent | BP-AS-10 | 1 mL |
| DNase I (2 U/μL) | BP-E04 | 50 μL |
| 10× DNase I Reaction Buffer | BP-AS-13 | 100 μL |
| RNase-free ddH2O | BP-AS-11 | 1 mL |
Note: The Control Template+ is for verification of in vitro transcription only and is not intended for downstream applications.
Performance data
1 Transcription Performance of T7 High-Efficiency Kit on templates of varying lengths (80 - 12,000 nt)
Description: To evaluate the transcription performance of the Biori T7 High-Efficiency Transcription Kit using DNA templates of varying lengths. A total of 10 templates ranging from 80 nt to 12,000 nt were transcribed under identical reaction conditions, and the resulting RNA products were analyzed by agarose gel electrophoresis for integrity.
Conclusion: The Biori T7 High-Efficiency Transcription Kit demonstrated broad template adaptability, successfully transcribing DNA templates across a wide size range (80 nt - 12,000 nt) with high RNA integrity, confirming its suitability for diverse template sizes.
2 Yield Comparison with Suppliers (UTP and N1-Me-pUTP)
Description: To compare the transcription performance of the Biori T7 High-Efficiency Transcription Kit with four supplier kits, using both natural and modified nucleotides. Transcription reactions were set up with the same DNA template under identical conditions, and yields were compared across kits using either UTP (natural) or N1-Me-pUTP (modified) as the nucleotide substrate.
Conclusion: The Biori T7 High-Efficiency Transcription Kit showed higher yields than all four supplier kits under modified (N1-Me-pUTP) nucleotide conditions and comparable or higher yields under natural (UTP) nucleotide conditions, highlighting its broad substrate adaptability.
Shipping and storage
| Condition | Recommendation |
|---|---|
| Storage | Store at -20±5°C. |
| Shipping | Ship frozen on dry ice or with validated frozen cold-chain packaging that maintains the specified frozen storage condition. |
| Handling | Avoid repeated freeze-thaw cycles; mix thoroughly after thawing where applicable. |
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