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Product Catalog
mRNA Co-transcription and Synthesis Kit, BP-07, supports co-transcriptional synthesis of capped mRNA from T7-promoter DNA templates. The workflow uses T7 RNA polymerase, NTP substrates, and cap analogs to generate 5′-capped RNA according to the instruction manual.
mRNA Co-transcription and Synthesis Kit, BP-07
USD
$0.00
BP-07-50
Features
- Kit format for RNA or mRNA synthesis workflow steps.
- Supplied with workflow-specific enzymes, buffers, nucleotides, or related reagents listed in the instruction manual.
- Designed for in vitro transcription, co-transcriptional synthesis, or circular RNA synthesis workflows as applicable.
Application
- RNA and mRNA synthesis workflow development.
- Preparation of RNA products using the protocol and components described in the instruction manual.
Components
| Components | Cat. No. | Volume |
|---|---|---|
| ATP Solution (100mM) | BP-AS-01 | 100uL |
| GTP Solution (100mM) | BP-AS-02 | 100uL |
| CTP Solution (100mM) | BP-AS-03 | 100uL |
| UTP Solution (100mM) | BP-AS-04 | 100uL |
| N1-Me-p UTP Solution (100mM) | BP-AS-07 | 100uL |
| T7RNA Polymerase | BP-E01 | 100uL |
| RNase Inhibitor (40 U/uL) | BP-E02 | 100uL |
| Inorganic Pyrophosphatase (0.1 U/uL) | BP-E03 | 100uL |
| DNase I (2 U/uL) | BP-E04 | 100uL |
| Cap AG (3’Acm)(100mM) | BP-AS-38 | 100uL |
| 10× Co-transcription Buffer | BP-AS-31 | 100uL |
| Precipitation Reagent | BP-AS-10 | 1mL |
| RNase-free ddH2O | BP-AS-11 | 1mL |
Shipping and Storage
| Condition | Recommendation |
|---|---|
| Storage | Store at -20±5°C. |
| Shipping | Ship frozen on dry ice or with validated frozen cold-chain packaging that maintains the specified frozen storage condition. |
| Handling | Avoid repeated freeze-thaw cycles; mix thoroughly after thawing where applicable. |
Data
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