CircRNA Synthesis Kit, BP-13
Kit for circular RNA synthesis workflows.
Product overview
The CircRNA Synthesis Kit (BP-13) is designed for circRNA synthesis using the components and workflow described in the instructions for use. It can be used to prepare covalently closed circRNA from a DNA template and is suitable for research and process-development applications.
Features and applications
- This kit is suitable for preparing circRNA based on a group I intron self-splicing mechanism.
- The kit provides the relevant transcription enzymes, buffers, nucleotides, and reagents for circRNA enrichment.
- Suitable for workflows including in vitro transcription and circRNA synthesis.
- The synthesized circRNA can be used directly in subsequent expression-efficiency studies;
- Supports early-stage research and development of circRNA-based vaccines and therapeutics;
- Suitable for mechanistic studies of circRNA in areas such as gene regulation and disease development.
Components
| Components | Cat. No. | Volume |
|---|---|---|
| ATP Solution(100 mM) | BP-AS-01 | 100 μL |
| GTP Solution(100 mM) | BP-AS-02 | 100 μL |
| CTP Solution(100 mM) | BP-AS-03 | 100 μL |
| UTP Solution(100 mM) | BP-AS-04 | 100 μL |
| T7 RNA Polymerase | BP-E01 | 100 μL |
| RNase Inhibitor(40 U/μL) | BP-E02 | 100 μL |
| Inorganic Pyrophosphatase(0.1 U/μL) | BP-E03 | 100 μL |
| 10×Transcription Buffer | BP-AS-08 | 100 μL |
| Precipitation Reagent | BP-AS-10 | 1 mL |
| DNase I (2 U/μL) | BP-E04 | 50 μL |
| 10×DNase I Reaction Buffer | BP-AS-13 | 100 μL |
| RNase-free ddH2O | BP-AS-11 | 1 mL |
| 10×Circular Buffer | BP-AS-30 | 1 mL |
| RNase R(20 U/μL) | BP-E08 | 50 μL |
| 10×RNase R Buffer | BP-AS-14 | 1 mL |
| RNase R Dilution Buffer | BP-AS-15 | 1 mL |
Performance data
1 Complete workflow of CircRNA Synthesis Kit
Description: To demonstrate the complete workflow of the Biori CircRNA Synthesis Kit, from DNA template to purified circular RNA. The kit provides all necessary components, including NTPs, transcription buffer, and enzyme mix for in vitro transcription, as well as circularization buffer and RNase R for linear RNA removal via Group I intron self-splicing. The products at each stage—preRNA (linear transcription product), circRNA (after circularization), and circRNA after RNase R digestion—were analyzed by agarose gel electrophoresis to confirm successful circularization and linear RNA removal.
Conclusion: The Biori CircRNA Synthesis Kit enables an integrated and efficient workflow from transcription through circularization and purification, as demonstrated by the successful generation of circRNA via Group I intron self-splicing and effective removal of linear precursors by RNase R digestion, yielding highly enriched circular RNA.
2 circRNA Expression in 293T Cells
Description: To evaluate the expression durability of circRNA prepared using the Biori CircRNA Synthesis Kit, 293T cells were transfected with purified circRNA. eGFP fluorescence was monitored at 24, 48, 72, and 168 h post-transfection to assess the duration and stability of transgene expression.
Conclusion: circRNA prepared using the Biori CircRNA Synthesis Kit mediated high-level eGFP expression in 293T cells at 24, 48, and 72 h post-transfection, as shown in Figure 2. Notably, clear fluorescence was still detectable at 168 h post-transfection, indicating sustained translational activity of the synthesized circRNA beyond the time points shown.
Shipping and storage
| Condition | Recommendation |
|---|---|
| Storage | Store at -20±5°C. |
| Shipping | Ship frozen on dry ice or with validated frozen cold-chain packaging that maintains the specified frozen storage condition. |
| Handling | Avoid repeated freeze-thaw cycles; mix thoroughly after thawing where applicable. |
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