AcuGenix™ LongR One-Step RT-PCR Kit

Closed-Tube Solution for High-Fidelity, Ultra-Long RNA Template Amplification

> 10 kb

Long RT-PCR targets

20 s / kb

Run finishes in 30 min

~50× fidelity

Proofreading hot-start polymerase

Product overview

The AcuGenix™ LongR One-Step RT-PCR Kit is a premium closed-tube amplification solution optimized for long RNA templates. It integrates reverse transcription (RT) and high-fidelity PCR into a single streamlined workflow via a synergistically engineered long-range enzyme cocktail (derived from directed evolution) and a pre-optimized buffer system tailored for long-amplicon synthesis. The kit eliminates intermediate purification or tube-opening steps, significantly minimizing aerosol contamination risks for high-throughput and clinical research workflows.

Features and applications

Features

Industry-Leading Long-Range Performance: Delivers stable synthesis and amplification of targets >10 kb, with an ultra-fast extension rate of 20 sec/kb. The full workflow (from reaction assembly to amplification completion) finishes within 30 minutes—representing a >2-fold efficiency improvement over conventional long-range one-step RT-PCR kits.
Ultra-High Fidelity: Paired with an antibody-blocked hot-start high-fidelity DNA polymerase that retains 3′→5′ exonuclease proofreading activity, offering ~50× higher fidelity than wild-type Taq DNA polymerase to minimize mismatch risks in long-amplicon synthesis.
Engineered Enzyme Synergy: The thermostable long-range reverse transcriptase exhibits exceptional processivity and RNA secondary structure unwinding activity, enabling efficient full-length cDNA synthesis across GC-rich or structurally complex regions that challenge conventional RT enzymes.
Optimized Specificity & Workflow Simplicity: The pre-optimized buffer is supplemented with long-range amplification enhancers and background suppressors to effectively inhibit primer-dimer formation and non-specific amplification, eliminating the need for laborious primer titration.
Exceptional Batch Consistency: Each batch is rigorously quality-controlled to be free of detectable RNase, DNase, and extraneous nucleic acid contamination. Inter-lot coefficient of variation (CV) for amplification efficiency and specificity is <5%, ensuring reproducible results across all production lots.

Applications

Full-genome sequencing of long RNA viruses (e.g., coronaviruses, influenza viruses, arboviruses, and emerging variants)
Full-length transcript analysis (mRNA isoform profiling, lncRNA/circRNA characterization)
Full-length cDNA synthesis for single-cell RNA-seq and spatial transcriptomics
Detection of RNA fusion genes and structural variants in long transcripts
Template preparation for long-read RNA sequencing (PacBio Iso-Seq, Oxford Nanopore Direct RNA Sequencing)

Components

Component	Specification/Notes
2×One Step Buffer	2×
One Step Enzyme Mix	/

Performance data

Amplification Assay for Targets of Different Lengths

BR3M321 AcuGenix™ LongR One-Step RT-PCR Kit: Figure 1. Amplification performance across 1–10.5 kb targets — **Figure 1. Amplification performance across 1–10.5 kb targets.** BR3M321 (AcuGenix™ LongR One-Step RT-PCR Kit) efficiently amplifies long targets ranging from 1 kb to 10.5 kb. For optimal product yield with longer fragments, moderately increasing the cycle number or template input is recommended.

Note: BR3M321 is the catalog number for this kit. The >10 kb amplification capacity covers the full genome length of most long RNA viruses and full-length transcripts.

Amplification Kinetics Assay

BR3M321 AcuGenix™ LongR One-Step RT-PCR Kit: Figure 2. Extension rate evaluation for 1–10.5 kb targets — **Figure 2. Extension rate evaluation for 1–10.5 kb targets.** The elongation rate is 20 sec/kb for targets up to 8 kb, with yield decreasing as fragment length increases. For fragments >5 kb, an extension time of 1 min/kb is recommended to balance amplification efficiency and product integrity.

Note: In long-range amplification, extension time should be dynamically adjusted based on target length to prevent incomplete products caused by insufficient extension.

Sensitivity Assay

BR3M321 AcuGenix™ LongR One-Step RT-PCR Kit: Figure 3. Template input titration from 10 pg to 1 μg — **Figure 3. Template input titration from 10 pg to 1 μg.** BR3M321 achieves a robust limit of detection down to 10 pg of total RNA, enabling reliable amplification of long targets from trace RNA samples. This performance supports demanding applications including low-input and rare transcript analysis.

Note: 10 pg of high-abundance total RNA corresponds to approximately 1–10 transcript copies, making the kit suitable for long-amplicon synthesis from single-cell or limited clinical specimens.