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Product Catalog
Sf9 & AcNPV Residual DNA Detection Kit (qPCR) is a 100-reaction BIORI qPCR kit for residual Sf9 cell and AcNPV DNA detection in insect cell-baculovirus workflows. It uses probe-based qPCR chemistry for conserved Sf9 cell and AcNPV genes. The kit includes internal control material and six concentration levels of Sf9 and AcNPV calibrators for vaccine quality analysis workflows.
Sf9 & AcNPV Residual DNA Detection Kit (qPCR), BP-QN21
USD
$0.00
BP-QN21-100
Features
- Detects residual DNA from Sf9 cells and AcNPV in one qPCR kit workflow.
- Targets conserved genes of Sf9 cells and AcNPV using probe-based qPCR chemistry.
- Includes an internal control to monitor extraction and amplification.
- Contains UDG to help prevent carryover contamination from amplification products.
- Includes Sf9 and AcNPV calibrators across six concentration levels.
- Supports residual DNA quantitation for insect cell-baculovirus expression system products.
- Supplied in a kit format suitable for real-time PCR quality analysis workflows.
Application
- Residual Sf9 cell DNA detection.
- Residual AcNPV DNA detection.
- Genetic engineering vaccine quality analysis using insect cell-baculovirus expression systems.
- Process development and quality analysis for products produced in baculovirus expression systems.
- qPCR workflows using supported real-time PCR instruments.
Components
| Component | Specification |
|---|---|
| Sf9 & AcNPV qPCR MIX | 1.0 mL x 2 tubes |
| Sf9 & AcNPV Internal Control | 1.0 mL x 1 tube |
| Sf9 & AcNPV Negative Control | 1.0 mL x 2 tubes |
| Sf9 & AcNPV Calibrator ST1 | 0.5 mL x 1 tube; Sf9: 3 fg/uL; AcNPV: 3 copies/uL |
| Sf9 & AcNPV Calibrator ST2 | 0.5 mL x 1 tube; Sf9: 30 fg/uL; AcNPV: 3 x 10^1 copies/uL |
| Sf9 & AcNPV Calibrator ST3 | 0.5 mL x 1 tube; Sf9: 300 fg/uL; AcNPV: 3 x 10^2 copies/uL |
| Sf9 & AcNPV Calibrator ST4 | 0.5 mL x 1 tube; Sf9: 3 pg/uL; AcNPV: 3 x 10^3 copies/uL |
| Sf9 & AcNPV Calibrator ST5 | 0.5 mL x 1 tube; Sf9: 30 pg/uL; AcNPV: 3 x 10^4 copies/uL |
| Sf9 & AcNPV Calibrator ST6 | 0.5 mL x 1 tube; Sf9: 300 pg/uL; AcNPV: 3 x 10^5 copies/uL |
Shipping and Storage
| Condition | Recommendation |
|---|---|
| Storage | Store protected from light at <= -20°C. Validity period: 24 months. |
| Shipping | Ship frozen or under validated cold-chain conditions that maintain frozen storage. |
| Handling | Avoid repeated freeze-thaw cycles; do not exceed 10 freeze-thaw cycles. |
Data
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