Sf9 & AcNPV Residual DNA Detection Kit (qPCR), BP-QN21
qPCR kit for Sf9 and AcNPV residual DNA detection.
Product overview
Sf9 & AcNPV Residual DNA Detection Kit (qPCR) is a 100-reaction BIORI qPCR kit for residual Sf9 cell and AcNPV DNA detection in insect cell-baculovirus workflows. It uses probe-based qPCR chemistry for conserved Sf9 cell and AcNPV genes. The kit includes internal control material and six concentration levels of Sf9 and AcNPV calibrators for vaccine quality analysis workflows.
Features and applications
- Detects residual DNA from Sf9 cells and AcNPV in one qPCR kit workflow.
- Targets conserved genes of Sf9 cells and AcNPV using probe-based qPCR chemistry.
- Includes an internal control to monitor extraction and amplification.
- Contains UDG to help prevent carryover contamination from amplification products.
- Includes Sf9 and AcNPV calibrators across six concentration levels.
- Supports residual DNA quantitation for insect cell-baculovirus expression system products.
- Supplied in a kit format suitable for real-time PCR quality analysis workflows.
- Residual Sf9 cell DNA detection.
- Residual AcNPV DNA detection.
- Genetic engineering vaccine quality analysis using insect cell-baculovirus expression systems.
- Process development and quality analysis for products produced in baculovirus expression systems.
- qPCR workflows using supported real-time PCR instruments.
Components
| Component | Specification |
|---|---|
| Sf9 & AcNPV qPCR MIX | 1.0 mL × 2 tubes |
| Sf9 & AcNPV Internal Control | 1.0 mL × 1 tube |
| Sf9 & AcNPV Negative Control | 1.0 mL × 2 tubes |
| Sf9 & AcNPV Calibrator ST1 | 0.5 mL × 1 tube; Sf9: 3 fg/µL; AcNPV: 3 copies/µL |
| Sf9 & AcNPV Calibrator ST2 | 0.5 mL × 1 tube; Sf9: 30 fg/µL; AcNPV: 3×10¹ copies/µL |
| Sf9 & AcNPV Calibrator ST3 | 0.5 mL × 1 tube; Sf9: 300 fg/µL; AcNPV: 3×10² copies/µL |
| Sf9 & AcNPV Calibrator ST4 | 0.5 mL × 1 tube; Sf9: 3 pg/µL; AcNPV: 3×10³ copies/µL |
| Sf9 & AcNPV Calibrator ST5 | 0.5 mL × 1 tube; Sf9: 30 pg/µL; AcNPV: 3×10⁴ copies/µL |
| Sf9 & AcNPV Calibrator ST6 | 0.5 mL × 1 tube; Sf9: 300 pg/µL; AcNPV: 3×10⁵ copies/µL |
Performance data
Performance Data
Sf9 & AcNPV Residual DNA Detection Kit (qPCR) Catalog No. BP-QN21-100
A product-specific validation study evaluated analytical measurement performance for Sf9 & AcNPV residual DNA. This selected upload version includes only the supported linearity and recovery results.
Key Performance at a Glance
| Performance parameter | Result |
|---|---|
| Linear range | Sf9: 1.5 fg/μL to 300 pg/μL AcNPV: 3.0 copies/μL to 3.00E+06 copies/μL |
| Acceptance range for recovery | 80%-120% |
Linearity
Observed in this validation study: for Sf9, across the evaluated range of 1.5 fg/μL to 300 pg/μL, the coefficient of determination (R2) was 0.99968 and the amplification efficiency was 96.940%.
Observed in this validation study: for AcNPV, across the evaluated range of 3.0 copies/μL to 3.00E+06 copies/μL, the coefficient of determination (R2) was 0.99946 and the amplification efficiency was 94.823%.
Recovery
Preparation of samples with different spiked amounts was conducted to evaluate the recovery. Observed recoveries ranged from 92.29% to 116.66% (acceptance range 80%-120%).
| Spiked amount (fg) | Sf9 | Spiked amount (copies) | AcNPV | ||||||
|---|---|---|---|---|---|---|---|---|---|
| Value (fg/μL) | Mean (fg/μL) | Elution volume (μL) | Recovery | Value (copies/μL) | Mean (copies/μL) | Elution volume (μL) | Recovery | ||
| 3.00E+02 | 3.55E+00 | 3.26E+00 | 1.00E+02 | 108.64% | 3.00E+02 | 2.93E+00 | 2.77E+00 | 1.00E+02 | 92.29% |
| 3.07E+00 | 2.53E+00 | ||||||||
| 3.16E+00 | 2.84E+00 | ||||||||
| 3.00E+03 | 3.01E+01 | 2.97E+01 | 1.00E+02 | 98.83% | 3.00E+03 | 3.12E+01 | 2.89E+01 | 1.00E+02 | 96.22% |
| 2.88E+01 | 2.71E+01 | ||||||||
| 3.00E+01 | 2.83E+01 | ||||||||
| 3.00E+04 | 3.37E+02 | 3.34E+02 | 1.00E+02 | 111.44% | 3.00E+04 | 3.06E+02 | 3.28E+02 | 1.00E+02 | 109.47% |
| 3.12E+02 | 3.04E+02 | ||||||||
| 3.54E+02 | 3.75E+02 | ||||||||
| 3.00E+05 | 3.03E+03 | 3.05E+03 | 1.00E+02 | 101.71% | 3.00E+05 | 3.09E+03 | 3.18E+03 | 1.00E+02 | 106.11% |
| 3.06E+03 | 3.20E+03 | ||||||||
| 3.06E+03 | 3.27E+03 | ||||||||
| 3.00E+06 | 3.36E+04 | 3.19E+04 | 1.00E+02 | 106.23% | 3.00E+06 | 3.27E+04 | 3.50E+04 | 1.00E+02 | 116.66% |
| 3.08E+04 | 3.71E+04 | ||||||||
| 3.11E+04 | 3.52E+04 | ||||||||
Recovery = Mean × Elution volume ÷ Spiked amount × 100%.
Performance data shown here were obtained with the reference materials, extraction workflows, instruments and acceptance criteria used in BIORI’s product-specific validation study. Users should verify performance in their own sample matrix and workflow.
Shipping and storage
| Condition | Recommendation |
|---|---|
| Storage | Store protected from light at <= -20°C. Validity period: 24 months. |
| Shipping | Ship frozen or under validated cold-chain conditions that maintain frozen storage. |
| Handling | Avoid repeated freeze-thaw cycles; do not exceed 10 freeze-thaw cycles. |
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