T7 High-Efficiency Transcription Kit, BP-01
High-efficiency IVT kit for RNA synthesis from T7 templates.
Product overview
T7 High-Efficiency Transcription Kit, BP-01, is an in vitro transcription kit for producing RNA from DNA templates containing a T7 promoter. It supports RNA and mRNA synthesis workflows using natural or modified nucleotides, and can be used for co-transcriptional capping when suitable cap analogs are included according to the protocol.
Features and applications
- Kit format for RNA or mRNA synthesis workflow steps.
- Supplied with workflow-specific enzymes, buffers, nucleotides, or related reagents listed in the instruction manual.
- Designed for in vitro transcription, co-transcriptional synthesis, or circular RNA synthesis workflows as applicable.
- RNA and mRNA synthesis workflow development.
- Preparation of RNA products using the protocol and components described in the instruction manual.
Components
| Components | Cat. No. | Volume |
|---|---|---|
| ATP Solution (100 mM) | BP-AS-01 | 100 μL |
| GTP Solution (100 mM) | BP-AS-02 | 100 μL |
| CTP Solution (100 mM) | BP-AS-03 | 100 μL |
| UTP Solution (100 mM) | BP-AS-04 | 100 μL |
| T7 RNA Polymerase | BP-E01 | 100 μL |
| RNase Inhibitor (40 U/μL) | BP-E02 | 100 μL |
| Inorganic Pyrophosphatase (0.1 U/μL) | BP-E03 | 100 μL |
| 10× Transcription Buffer | BP-AS-08 | 100 μL |
| Control Template+ | BP-AS-09 | 5 μL |
| Precipitation Reagent | BP-AS-10 | 1 mL |
| DNase I (2 U/μL) | BP-E04 | 50 μL |
| 10× DNase I Reaction Buffer | BP-AS-13 | 100 μL |
| RNase-free ddH2O | BP-AS-11 | 1 mL |
Performance data
1 Transcription Performance of T7 High-Efficiency Kit on templates of varying lengths (80 - 12,000 nt)
Description: To evaluate the transcription performance of the Biori T7 High-Efficiency Transcription Kit using DNA templates of varying lengths. A total of 10 templates ranging from 80 nt to 12,000 nt were transcribed under identical reaction conditions, and the resulting RNA products were analyzed by agarose gel electrophoresis for integrity.
Conclusion: The Biori T7 High-Efficiency Transcription Kit demonstrated broad template adaptability, successfully transcribing DNA templates across a wide size range (80 nt - 12,000 nt) with high RNA integrity, confirming its suitability for diverse template sizes.
2 Yield Comparison with Suppliers (UTP and N1-Me-pUTP)
Description: To compare the transcription performance of the Biori T7 High-Efficiency Transcription Kit with four supplier kits, using both natural and modified nucleotides. Transcription reactions were set up with the same DNA template under identical conditions, and yields were compared across kits using either UTP (natural) or N1-Me-pUTP (modified) as the nucleotide substrate.
Conclusion: The Biori T7 High-Efficiency Transcription Kit demonstrated superior transcription yields compared with all four supplier kits, under both natural (UTP) and modified (N1-Me-pUTP) nucleotide conditions, highlighting its broad substrate adaptability.
Shipping and storage
| Condition | Recommendation |
|---|---|
| Storage | Store at -20±5°C. |
| Shipping | Ship frozen on dry ice or with validated frozen cold-chain packaging that maintains the specified frozen storage condition. |
| Handling | Avoid repeated freeze-thaw cycles; mix thoroughly after thawing where applicable. |
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